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作者

Lin-Lin Gao Fu-Rong Li Peng Jiao Ming-Feng Yang Xiao-Jun Zhou Yan-Hong Si Wen-Jian Jiang Ting-Ting Zheng

作者单位

Peng Jiao, Ming-Feng Yang, Institute of Basic Medical Sciences, Taishan Medical University, Taian 271000, Shandong Province, China Lin-Lin Gao, Yan-Hong Si, Department of Pathology and Pathophysiology, Taishan Medical University, Taian 271000, Shandong Province, China Fu-Rong Li, School of Pharmaceutical Science, Taishan Medical University, Taian 271000, Shandong Province, China Xiao-Jun Zhou, Wen-Jian Jiang, Ting-Ting Zheng, Department of Clinical Medicine, Taishan Medical University, Taian 271000, Shandong Province, China

刊名

World Journal of Gastroenterology

年份

2011

卷号

Vol.17 No.39

页码

4389-4395

ISSN

1007-9327

关键词

CyclinB1/CDK1 Cell cycle arrest Caspase-3 Ca2+ Cytochrome C

摘要

AIM: To investigate the anti-tumor effects of Paris chinensis dioscin (PCD) and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 cells. METHODS: Cell viability was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Cell apoptosis was evaluated by flow cytometry and laser scanning confocal microscope (LSCM) using Annexin-V/propidium iodide (PI) staining, and the cell cycle was evaluated using PI staining with flow cytometr...更多

AIM: To investigate the anti-tumor effects of Paris chinensis dioscin (PCD) and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 cells. METHODS: Cell viability was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Cell apoptosis was evaluated by flow cytometry and laser scanning confocal microscope (LSCM) using Annexin-V/propidium iodide (PI) staining, and the cell cycle was evaluated using PI staining with flow cytometry. Intracellular calcium ions were detected under fluorescence microscope. The expression of cell cycle and apoptosis-related proteins cyclin B1, CDK1, cytochrome C and caspase-3 was measured by immunohistochemical staining. RESULTS: PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose- and time-dependent manner. After treatment of SGC-7901 cells with PCD, apoptosis appeared in SGC-7901 cells. Morphological changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining, and the cell number of the G0/G1 phase was decreased, while the number of cells in the G2/M phase was increased. Cell cycle-related proteins, such as cyclin B1 and CDK1, were all down-regulated, but caspase-3 and cytochrome C were up-regulated. Moreover, intracellular calcium accumulation occurred in PCD-treated cells. CONCLUSION: G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells.收起

学科

【SCI学科】GASTROENTEROLOGY & HEPATOLOGY 【ESI学科】CLINICAL MEDICINE

影响因子

2016年:3.365;2015年:2.787;2014年:2.369;2013年:2.433;2012年:2.547

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期刊

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