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作者

HUANLING WU SHUQUAN BIAN JINGXUE CHU XIAOYAN ZHONG HUI SUN BINGCHANG ZHANG ZHIMING LU

作者单位

Clinical Laboratory of Jinan Central Hospital, Jinan, Shandong 250013 Shandong Center For Clinical Laboratories, Jinan, Shandong 250021, P.R. China Clinical Laboratory of the Eighth Hospital in Jinan, Jinan, Shandong 250014 Clinical Laboratory of Xintai Hospital Affiliated to Taishan Medical University, Jinan, Shandong 271200 Clinical Laboratory of Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021

刊名

Exp Ther Med

年份

2013

卷号

Vol.5 No.5

页码

1332-1338

关键词

chronic cytogenetic dysplasia immunology molecular genetic myelodysplastic myeloproliferative neoplasms

摘要

The aim of the present study was to investigate the characteristics of the four subtypes of myelodysplastic/myeloproliferative neoplasms (MDS/MPNs) in order to improve current knowledge and to aid their diagnosis. A total of 53 cases of MDS/MPNs were analyzed using routine blood cell analysis and morphological, cytogenetic and molecular genetic characteristics were investigated. Numerical data for several groups were compared using a single-factor analysis of variance. The Student-Newman-Keuls t...更多

The aim of the present study was to investigate the characteristics of the four subtypes of myelodysplastic/myeloproliferative neoplasms (MDS/MPNs) in order to improve current knowledge and to aid their diagnosis. A total of 53 cases of MDS/MPNs were analyzed using routine blood cell analysis and morphological, cytogenetic and molecular genetic characteristics were investigated. Numerical data for several groups were compared using a single-factor analysis of variance. The Student-Newman-Keuls test was used to compare the means of two groups. The proportions were compared using a Chi-square test or Fisher's exact test. Analysis of the patients with MDS/MPNs revealed that 46 patients (86.8%) had paleness and fatigue, and blood analysis revealed hemoglobin (Hb) levels of 83.1±24.6 g/l, a white blood cell (WBC) count of 19.8±8.1×109/l and a platelet (PLT) count of 158.7±108.2×1012/l. Immature neutrophils and monocytes were identified in the peripheral blood at levels of 0.058±0.031 and 0.152±0.034%, respectively. There were 23 cases (43.4%) with dyserythropoiesis and 36 cases (67.9%) had dysgranulopoiesis. Fifteen cases were immunologically characterized using flow cytometry (FCM), of which 13 cases showed abnormalities on blasts and myelocytes. Karyotyping was performed in 27 cases of MDS/MPN and 12 (44.4%) were identified as abnormal. In 23 cases, testing for BCR/ABL1, AML-ETO, CBF-MYH11A, PML-RARA, E2A-PBX1, TEL-AML1, SIL-TAL1 returned negative results. The JAK2V617F mutation was positive in one of five cases. The majority of MDS/MPN cases had anemia, cytosis, low-grade blasts and immature neutrophils in the peripheral blood and dysplasia in the bone marrow. Immunological abnormalities and abnormal karyotypes occurred frequently in MDS/MPNs and although there were no statistical differences between the four subtypes, these were able to aid diagnosis. No specific molecular abnormalities were identified in MDS/MPNs.收起

影响因子

2016年:1.261;2015年:1.28;2014年:1.269;2013年:0.941;2012年:0.344

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期刊

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