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作者

芮泽 张喜善 张辉 范克伟 王德峰

作者单位

泰山医学院附属医院骨一科

会议名称

第21届中国康协肢残康复学术年会暨第二届“泰山杯”全国骨科青年科技创新论坛

会议录

第21届中国康协肢残康复学术年会暨第二届“泰山杯”全国骨科青年科技创新论坛论文集

会议地点

山东泰安

召开年

2012

摘要

Bone healing depends on vascular endothelial growth factor (VEGF) secretion from osteoblasts to promote angiogenesis. We examined the influence of the tyrosine 321 site of G protein - coupled receptor kinase interacting protein 1 (GIT1) on platelet -derived growth factor (PDGF) -induced VEGF synthesis in vitro and on bone healing in vivo. Cultured osteoblasts were prepared from calvaria of 1 -2 -day -old rats. The phospho -activation of extracellular signal -regulated kinases 1/2 (ERK1/2), GIT1,...更多

Bone healing depends on vascular endothelial growth factor (VEGF) secretion from osteoblasts to promote angiogenesis. We examined the influence of the tyrosine 321 site of G protein - coupled receptor kinase interacting protein 1 (GIT1) on platelet -derived growth factor (PDGF) -induced VEGF synthesis in vitro and on bone healing in vivo. Cultured osteoblasts were prepared from calvaria of 1 -2 -day -old rats. The phospho -activation of extracellular signal -regulated kinases 1/2 (ERK1/2), GIT1, the interaction between GIT1 and ERK1/2, and VEGF mRNA expression were measured in response to PDGF. In addition, PDGF was applied following pretreatment with the MEK1/2 inhibitor PD98059 or the Src inhibitor PP2. We mutated tyrosines 293 or 321 of GIT1 individually to phenylalanine (mutants GIT1Y293F and GIT1Y321F) and incorporated these mutants and native G1T1 into lentivirus vectors. The relationship between GIT1 and ERK1/2, and VEGF mRNA expression in cultured osteoblasts were detected after infection with GIT1WT - , GIT1Y293F- and GJT1Y321 K-expressing lentivirus in response to PDGF. Bone healing and expression of VEGF and the angiogenic marker PEC AM - 1 were evalualed alter infection at the fracture site. Activation of ERK1/2 by phosphorylation, CIT1 tyrosine phosphoryla-tion, GIT! - ERK1/2 interaction, and VEGF mRNA expression were all significantly increased in osteohlasts after PDGF stimulation, but all responses were dramatically inhibited by pretreatment with PD98059. Tyrosine phosphorylation, GIT1 interaction with ERK1/2, and VEGF mRNA expression were dramatically inhibited by pretreatment with PP2 or infection with GIT1Y321F -expressing lentivirus. Expression of VEGF and PECAM - 1 was significantly lower at the fracture sites infected with CIT1 Y321F -expressing lentivirus and bone healing was significantly-delayed compared to fracture sites infected with GIT1WT. In conclusion, tyrosine 321 of GIT1 is a critical phos-phorylation site for GIT1 interaction with ERK1/2, regulation of ERK1/2 activation, VEGF expression and angio-genesis at the fracture site. Furthermore, GIT1 is a seminal signaling protein regulating bone healing.收起

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