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作者

Yong Zhang Shuangli Zhu Dongmei Yan Guiyan Liu Ruyin Bai Dongyan Wang Li Chen Hui Zhu Hongqiu An Olen Kew Wenbo Xu

作者单位

WHO WPRO Regional Polio Reference Laboratory and State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China. Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. Taishan Medical University, Taishan City, People's Republic of China. Jilin Center for Disease Control and Prevention, Changchun, People's Republic of China.

刊名

PLoS ONE

年份

2010

卷号

Vol.5 No.12

页码

1-11

ISSN

1932-6203

摘要

Background: Ten uncommon natural type 3/type 2 intertypic poliovirus recombinants were isolated from stool specimens from nine acute flaccid paralysis case patients and one healthy vaccinee in China from 2001 to 2008. Principal Findings: Complete genomic sequences revealed their vaccine-related genomic features and showed that their first crossover sites were randomly distributed in the 39 end of the VP1 coding region. The length of donor Sabin 2 sequences ranged from 55 to 136 nucleotides, whic...更多

Background: Ten uncommon natural type 3/type 2 intertypic poliovirus recombinants were isolated from stool specimens from nine acute flaccid paralysis case patients and one healthy vaccinee in China from 2001 to 2008. Principal Findings: Complete genomic sequences revealed their vaccine-related genomic features and showed that their first crossover sites were randomly distributed in the 39 end of the VP1 coding region. The length of donor Sabin 2 sequences ranged from 55 to 136 nucleotides, which is the longest donor sequence reported in the literature for this type of poliovirus recombination. The recombination resulted in the introduction of Sabin 2 neutralizing antigenic site 3a (NAg3a) into a Sabin 3 genomic background in the VP1 coding region, which may have been altered by some of the type 3-specific antigenic properties, but had not acquired any type 2-specific characterizations. NAg3a of the Sabin 3 strain seems atypical; other wild-type poliovirus isolates that have circulated in recent years have sequences of NAg3a more like the Sabin 2 strain. Conclusions: 10 natural type 3/type 2 intertypic VP1 capsid-recombinant polioviruses, in which the first crossover sites were found to be in the VP1 coding region, were isolated and characterized. In spite of the complete replacement of NAg3a by type 2-specific amino acids, the serotypes of the recombinants were not altered, and they were totally neutralized by polyclonal type 3 antisera but not at all by type 2 antisera. It is possible that recent type 3 wild poliovirus isolates may be a recombinant having NAg3a sequences derived from another strain during between 1967 and 1980, and the type 3/type 2 recombination events in the 39 end of the VP1 coding region may result in a higher fitness.收起

学科

【SCI学科】MULTIDISCIPLINARY SCIENCES 【ESI学科】Multidisciplinary

影响因子

2016年:2.806;2015年:3.057;2014年:3.234;2013年:3.534;2012年:3.73

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