Effect of radiation on cytotoxicity, apoptosis and cell cycle arrest of human osteosarcoma MG-63 induced by a ruthenium complex.

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作者: Si-Hong Liu Jian-Hua Zhao Kun-Kang Deng Yong Wu Jian-Wei Zhu Qing-Hua Liu Hui-Hua Xu Hai-Feng Wu Xin-Yan Li Jian-Wei Wang Qi-Feng Guo

作者单位: a Department of Orthopaedics, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, PR China Department of Oncology, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, PR China Department of Orthopaedics, Taishan City People’s Hospital, Taishan 529200, PR China

关键词: Apoptosis Cell cycle arrest Cytotoxicity in vitro Mitochondrial membrane potential Reactive oxygen species Ruthenium(II) complex

摘要: Radiation has large influence on the cytotoxicity, apoptosis and cell cycle arrest. The bioactivity of ruthenium complex [Ru 2 ] 2 imidazo[4,5-f][1,10]phenanthroline) was investigated in the absence and presence of radiation. The cytotoxicity of Ru1 against MG-63 cells was evaluated by CCK-8 method. Ru1 shows high cytotoxicity upon radiation. Radiation can enhance the cytotoxicity of Ru1 on MG-63 cells. The apoptosis was studied by Hoechst 33258 staining method and flow cytometry. The reactive ...更多; Radiation has large influence on the cytotoxicity, apoptosis and cell cycle arrest. The bioactivity of ruthenium complex [Ru 2 ] 2 imidazo[4,5-f][1,10]phenanthroline) was investigated in the absence and presence of radiation. The cytotoxicity of Ru1 against MG-63 cells was evaluated by CCK-8 method. Ru1 shows high cytotoxicity upon radiation. Radiation can enhance the cytotoxicity of Ru1 on MG-63 cells. The apoptosis was studied by Hoechst 33258 staining method and flow cytometry. The reactive oxygen species, mitochondrial membrane potential, cell cycle arrest and western blot analysis were investigated in detail. The complex induces the apoptosis in MG-63 cells through ROS-mediated mitochondrial dysfunction pathway.收起

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