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作者

Du Hui Shi Juan Wang Ming An Shuhong Guo Xingjing Wang Zhaojin

作者单位

Department of Histology and Embryology, Taishan Medical University, Taian, 271000, China. Department of Human Anatomy, Taishan Medical University, Taian, 271000, China. Department of Physiology, Taishan Medical University, Taian, 271000, China. Department of Human Anatomy, Taishan Medical University, Taian, 271000, China. zjwang@tsmc.edu.cn.

刊名

Journal of neuroinflammation

年份

2018

卷号

Vol.15 No.1

页码

280

ISSN

1742-2094

关键词

Chronic constriction injury Differentially expressed genes Dorsal horn RNA sequencing

分类号

616.8

摘要

BACKGROUND: Neuropathic pain is caused by damage to the nervous system, resulting in aberrant pain, which is associated with gene expression changes in the sensory pathway. However, the molecular mechanisms are not fully understood. METHODS: Wistar rats were employed for the establishment of the chronic constriction injury models. Using the Illumina HiSeq 4000 platform, we examined differentially expressed genes in the rat dorsal horn by RNA sequencing between CCI and control groups. Then, en...更多

BACKGROUND: Neuropathic pain is caused by damage to the nervous system, resulting in aberrant pain, which is associated with gene expression changes in the sensory pathway. However, the molecular mechanisms are not fully understood. METHODS: Wistar rats were employed for the establishment of the chronic constriction injury models. Using the Illumina HiSeq 4000 platform, we examined differentially expressed genes in the rat dorsal horn by RNA sequencing between CCI and control groups. Then, enrichment analyses were performed for these DEGs using Gene Ontology function, Kyoto Encyclopedia of Genes and Genomes pathway, Hierarchical Cluster, and protein-protein interaction network. RESULTS: A total of 63 DEGs were found significantly changed with 56 upregulated and 7 downregulated at 14 days after CCI. Quantitative reverse-transcribed PCR verified changes in 13 randomly selected DEGs. GO and KEGG biological pathway analyses showed that the upregulated DEGs were mostly enriched in immune response-related biological processes, as well as 14 immune- and inflammation-related pathways. The downregulated DEGs were enriched in inactivation of mitogen-activated protein kinase activity. PPI network analysis showed that Cd68, C1qc, C1qa, Laptm5, and Fcgr3a were crucial nodes with high connectivity degrees. Most of these genes which have previously been linked to immune and inflammation-related pathways have not been reported in neuropathic pain . CONCLUSIONS: Our results revealed that immune and defense pathways may contribute to the generation of neuropathic pain after CCI. These mRNAs may represent new therapeutic targets for the treatment of neuropathic pain.收起

学科

【SCI学科】IMMUNOLOGY;NEUROSCIENCES

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