TaiShan Med Coll, Dept Neurol, Affiliated Hosp, Tai An, Shandong, Peoples R China 增强组织信息的名称 Taishan Medical UniversitySun Yat Sen Univ, Dept Neurol, Affiliated Hosp 1, Guangzhou 510275, Guangdong, Peoples R China 增强组织信息的名称 Sun Yat Sen University
Objective: Intracellular chloride concentration of a given cell within the central nervous system is determined by a delicate balance between the Cl- extrusion system and the Cl- accumulation system. In this study, we aim to investigate the expression profiles of cation-chloride cotransporters (CCCs) mRNA in the mice model of lithium-pilocarpine induced status epilepticus (PISE) and the possible relationships between CCCs and epileptogenesis.Methods: Male adult Balb/c mice were randomly divided ...更多
Objective: Intracellular chloride concentration of a given cell within the central nervous system is determined by a delicate balance between the Cl- extrusion system and the Cl- accumulation system. In this study, we aim to investigate the expression profiles of cation-chloride cotransporters (CCCs) mRNA in the mice model of lithium-pilocarpine induced status epilepticus (PISE) and the possible relationships between CCCs and epileptogenesis.Methods: Male adult Balb/c mice were randomly divided into two groups: the PISE group and the control. We used the mice of PISE group to establish the animal model of PISE and the PISE group could be subdivided into three time points: 1 d, 14 d and 45 d. Real-time fluorescence quantitative PCR (polymerase chain reaction) was used to explore the expression of CCCs family members mRNA in CA1 region.Results: These CCCs members all showed distinctive expression patterns. The expression levels of NKCC1 (sodium potassium cation-chloride cotransporter 1), KCC1 (potassium cation-chloride cotransporter 1), KCC3 (potassium cation-chloride cotransporter 3) and CIP1 (CCC-interacting protein type 1)mRNA were up-regulated while those of KCC2 (potassium cation-chloride cotransporter 2) and CCC9 (cation-chloride cotransporter) mRNA were down-regulated after PISE. The expression of KCC4 (potassium cation-chloride cotransporter 4) remained unchanged.Conclusions: These data lead us to conclude that CCCs expression as a whole is under very strict transcriptional control in this model. Deregulation of their expression might break the balance of intracellular and extracellular chloride concentration which contributed to the mechanism of hyperexcitability that led to seizures. This may be due to some form of genetic program activated in response to epileptic seizure in the brain and may highlight which biological pathways are modulated.收起
