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作者

Zong, CL Song, GH Yao, ST Li, LQ Yu, Y Feng, L Guo, SD Luo, T Qin, SC

作者单位

Taishan Med Univ, Inst Atherosclerosis, Shandong 271000, Peoples R China   增强组织信息的名称     Taishan Medical University Taishan Med Univ, Shandong Univ, Key Lab Atherosclerosis, Shandong 271000, Peoples R China   增强组织信息的名称     Taishan Medical University     Shandong University

刊名

Metabolism

年份

2012

卷号

Vol.61 No.6

页码

794-800

ISSN

0026-0495

关键词

APOLIPOPROTEIN-E TRANSFER PROTEIN MESSENGER-RNA METABOLISM INFLAMMATION RECEPTOR GENE ATHEROSCLEROSIS MECHANISM DISEASE

分类号

616.4

摘要

Hydrogen (dihydrogen; H) has an antiatherosclerotic effect in apolipoprotein (apo) E knockout mice. The goals of this study were to further characterize the effects of H on the content, composition, and biological activities of plasma lipoproteins in golden hamsters. Plasma analysis by enzymatic method and fast protein liquid chromatography showed that 4-week intraperitoneal injection of hydrogen-saturated saline remarkably decreased plasma total cholesterol and low-density lipoprotein (LDL) cho...更多

Hydrogen (dihydrogen; H) has an antiatherosclerotic effect in apolipoprotein (apo) E knockout mice. The goals of this study were to further characterize the effects of H on the content, composition, and biological activities of plasma lipoproteins in golden hamsters. Plasma analysis by enzymatic method and fast protein liquid chromatography showed that 4-week intraperitoneal injection of hydrogen-saturated saline remarkably decreased plasma total cholesterol and low-density lipoprotein (LDL) cholesterol levels in high-fat diet–fed hamsters. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of apolipoproteins from ultracentrifugally isolated plasma lipoproteins revealed a marked decrease of apo B100 and apo B48 in LDL. A profound decrease of apo E level in very low-density lipoprotein was also observed. Besides, we determined the functional quality of high-density lipoprotein (HDL) particles isolated from H-treated and control mice. H significantly improved HDL functionality assessed in 2 independent ways, namely, (1) stimulation of cholesterol efflux from macrophage foam cells by measuring HDL-induced [H]cholesterol efflux and (2) protection against LDL oxidation as a measure of Cu-induced thiobarbituric acid reactive substances formation. Administration of hydrogen-saturated saline decreases plasma LDL cholesterol and apo B levels and improves hyperlipidemia-injured HDL functions, including the capacity of enhancing cellular cholesterol efflux and playing antioxidative properties, in high-fat diet–fed hamsters.收起

影响因子

2016年:5.777;2015年:4.375;2014年:3.894;2013年:3.611;2012年:3.096

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