Taishan Med Univ, Affiliated Hosp, Dept Breast Surg, Tai An 27100, Shandong, Peoples R ChinaTaishan Med Univ, Affiliated Hosp, Dept Hematol, Tai An, Shandong, Peoples R ChinaTaishan Med Univ, Affiliated Hosp, Dept Clin Lab, Tai An, Shandong, Peoples R ChinaTaishan Med Univ, Affiliated Hosp, Med Examinat Ctr, Tai An, Shandong, Peoples R ChinaShanghai Jiao Tong Univ, Peoples Hosp 1, Dept Clin Lab, Shanghai, Peoples R ChinaTaishan Med Univ, Affiliated Hosp, Burn & Plast Dept, Tai An, Shandong, Peoples R China
刊名
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
Objective: This study aimed to observe the effects of phosphatidylinositol 3 kinase (PI3K) specific inhibitor LY294002 on CD3 monoclonal antibody (CD3mAb) activated T cells and Mtb-Ag activated gamma delta T cells, and to investigate the function of PI3K in TCR signal transduction pathway. Methods: Healthy human Peripheral blood mononuclear cell (PBMC) was isolated. CD3mAb, phorbol esters (PMA), ionomycin (IM), and Mtb-Ag were used to stimulate PBMC. Flow cytometry was used to detect the express...更多
Objective: This study aimed to observe the effects of phosphatidylinositol 3 kinase (PI3K) specific inhibitor LY294002 on CD3 monoclonal antibody (CD3mAb) activated T cells and Mtb-Ag activated gamma delta T cells, and to investigate the function of PI3K in TCR signal transduction pathway. Methods: Healthy human Peripheral blood mononuclear cell (PBMC) was isolated. CD3mAb, phorbol esters (PMA), ionomycin (IM), and Mtb-Ag were used to stimulate PBMC. Flow cytometry was used to detect the expression of CD69 in CD3(+) T cells and gamma delta T cells at different time. PBMC were pretreated by LY294002, followed by double staining with CD3PE/CD69 FITC and gamma delta PE/CD69 FITC. The CD69 was detected in CD3(+) T cells and in gamma delta T cells. The total number of cells cultured for 10 days was counted. Results: After stimulation by CD3mAb for 24 h, the expression of CD69 in both CD3(+) T cells and gamma delta T cells was both increased to about 56%. After stimulation by PMA and IM for 6 h, the expression of CD69 in both CD3(+) T cells and gamma delta T cells was increased to about 99%. After stimulation by Mtb-Ag for 24 h, the expression of CD69 in CD3(+) T cells was increased to 16.0%, while 75.2% in gamma delta T cells. The expression of CD69 in Mtb-Ag activated gamma delta T cells and proliferation of gamma delta T cells were obviously inhibited after pretreatment with LY294002. Conclusion: gamma delta T cells are specifically activated by Mtb-Ag, and its activation depends on TCR pathway. The activation effects of CD3mAb on T cells and Mtb-Ag on gamma delta T cells are both inhibited by LY294002.收起
