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作者

Wang, KQ Hou, YQ Li, QH Zhao, DP Duan, YC Ran, ZS Li, XQ

作者单位

Taishan Med Univ, Affiliated Hosp, Dept Breast Surg, Tai An 27100, Shandong, Peoples R China Taishan Med Univ, Affiliated Hosp, Dept Hematol, Tai An, Shandong, Peoples R China Taishan Med Univ, Affiliated Hosp, Dept Clin Lab, Tai An, Shandong, Peoples R China Taishan Med Univ, Affiliated Hosp, Med Examinat Ctr, Tai An, Shandong, Peoples R China Shanghai Jiao Tong Univ, Peoples Hosp 1, Dept Clin Lab, Shanghai, Peoples R China Taishan Med Univ, Affiliated Hosp, Burn & Plast Dept, Tai An, Shandong, Peoples R China

刊名

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY

年份

2017

卷号

Vol.10 No.5

页码

5538-5544

ISSN

1936-2625

关键词

gamma delta T cells CD69 molecules signal transduction LY294002 CD3mAb PMA IM Mtb-Ag

分类号

616.07

摘要

Objective: This study aimed to observe the effects of phosphatidylinositol 3 kinase (PI3K) specific inhibitor LY294002 on CD3 monoclonal antibody (CD3mAb) activated T cells and Mtb-Ag activated gamma delta T cells, and to investigate the function of PI3K in TCR signal transduction pathway. Methods: Healthy human Peripheral blood mononuclear cell (PBMC) was isolated. CD3mAb, phorbol esters (PMA), ionomycin (IM), and Mtb-Ag were used to stimulate PBMC. Flow cytometry was used to detect the express...更多

Objective: This study aimed to observe the effects of phosphatidylinositol 3 kinase (PI3K) specific inhibitor LY294002 on CD3 monoclonal antibody (CD3mAb) activated T cells and Mtb-Ag activated gamma delta T cells, and to investigate the function of PI3K in TCR signal transduction pathway. Methods: Healthy human Peripheral blood mononuclear cell (PBMC) was isolated. CD3mAb, phorbol esters (PMA), ionomycin (IM), and Mtb-Ag were used to stimulate PBMC. Flow cytometry was used to detect the expression of CD69 in CD3(+) T cells and gamma delta T cells at different time. PBMC were pretreated by LY294002, followed by double staining with CD3PE/CD69 FITC and gamma delta PE/CD69 FITC. The CD69 was detected in CD3(+) T cells and in gamma delta T cells. The total number of cells cultured for 10 days was counted. Results: After stimulation by CD3mAb for 24 h, the expression of CD69 in both CD3(+) T cells and gamma delta T cells was both increased to about 56%. After stimulation by PMA and IM for 6 h, the expression of CD69 in both CD3(+) T cells and gamma delta T cells was increased to about 99%. After stimulation by Mtb-Ag for 24 h, the expression of CD69 in CD3(+) T cells was increased to 16.0%, while 75.2% in gamma delta T cells. The expression of CD69 in Mtb-Ag activated gamma delta T cells and proliferation of gamma delta T cells were obviously inhibited after pretreatment with LY294002. Conclusion: gamma delta T cells are specifically activated by Mtb-Ag, and its activation depends on TCR pathway. The activation effects of CD3mAb on T cells and Mtb-Ag on gamma delta T cells are both inhibited by LY294002.收起

学科

【SCI学科】PATHOLOGY;ONCOLOGY 【ESI学科】CLINICAL MEDICINE

影响因子

2016年:1.706;2015年:1.581;2014年:1.891;2013年:1.783;2012年:2.242

文献类型

期刊

浏览量

19