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作者

Zhang, XH Song, X Wang, Y Lu, KN Bi, WK Shi, CH Deng, YX Wang, KQ

作者单位

Taishan Med Univ, Affiliated Hosp, Dept Endocrinol, 706 Taishan Rd, Tai An 271000, Shandong, Peoples R China Taishan Med Univ, Affiliated Hosp, Dept Clin Labs, Tai An 271000, Shandong, Peoples R China

刊名

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY

年份

2016

卷号

Vol.9 No.4

页码

4595-4600

ISSN

1936-2625

关键词

Hypothyroidism myocardium mir-124 IGF-1

分类号

616.07

摘要

Hypothyroidism is a common disorder of the endocrine system caused by reduced thyroid hormone synthesis in the thyroid gland. This study aimed to investigate the myocardial pathologic change and the interaction between mir-124 and IGF-1 expression in hypothyroid rats. Sixty SPF male Westar rats were randomly divided into 3 groups: hypothyroidism, L-T4 treatment and control group. After 12 weeks, serum levels of thyroid-stimulating hormone (TSH) and total thyroxin (TT4) were measured by immunoflu...更多

Hypothyroidism is a common disorder of the endocrine system caused by reduced thyroid hormone synthesis in the thyroid gland. This study aimed to investigate the myocardial pathologic change and the interaction between mir-124 and IGF-1 expression in hypothyroid rats. Sixty SPF male Westar rats were randomly divided into 3 groups: hypothyroidism, L-T4 treatment and control group. After 12 weeks, serum levels of thyroid-stimulating hormone (TSH) and total thyroxin (TT4) were measured by immunofluorescence. The morphological change of the myocardial tissue was observed by HIM staining. The expression of mir-124 and IGF-1 mRNA was determined by real-time quantitative PCR. The expression of IGF-1 protein was quantified by Western blot analysis. At week 2, the TSH level in hypothyroidism and L-T4 treatment groups was significantly higher compared with control group (P < 0.01), whereas the TT4 level in both groups was significantly lower (P < 0.01). At week 12, the TSH level in hypothyroidism group was significantly higher compared with L-T4 treatment and control group (P < 0.01), but the TT4 level in hypothyroidism group was significantly lower (P < 0.01). The mir-124 expression in hypothyroidism group was significantly higher compared with both L-T4 treatment and control group (P < 0.05). The expression of IGF-1 protein in hypothyroidism group was significantly lower compared with L-T4 treatment and control group (P < 0.05). The level of thyroid hormone was reduced in the thyroid gland in hypothyroid rats. IGF-1 expression was inhibited through increasing the myocardial mir-124 level.收起

学科

【SCI学科】PATHOLOGY;ONCOLOGY 【ESI学科】CLINICAL MEDICINE

影响因子

2016年:1.706;2015年:1.581;2014年:1.891;2013年:1.783;2012年:2.242

文献类型

期刊

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