Taishan Med Univ, Affiliated Hosp, Dept Endocrinol, 706 Taishan Rd, Tai An 271000, Shandong, Peoples R ChinaTaishan Med Univ, Affiliated Hosp, Dept Clin Labs, Tai An 271000, Shandong, Peoples R China
刊名
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
Hypothyroidism is a common disorder of the endocrine system caused by reduced thyroid hormone synthesis in the thyroid gland. This study aimed to investigate the myocardial pathologic change and the interaction between mir-124 and IGF-1 expression in hypothyroid rats. Sixty SPF male Westar rats were randomly divided into 3 groups: hypothyroidism, L-T4 treatment and control group. After 12 weeks, serum levels of thyroid-stimulating hormone (TSH) and total thyroxin (TT4) were measured by immunoflu...更多
Hypothyroidism is a common disorder of the endocrine system caused by reduced thyroid hormone synthesis in the thyroid gland. This study aimed to investigate the myocardial pathologic change and the interaction between mir-124 and IGF-1 expression in hypothyroid rats. Sixty SPF male Westar rats were randomly divided into 3 groups: hypothyroidism, L-T4 treatment and control group. After 12 weeks, serum levels of thyroid-stimulating hormone (TSH) and total thyroxin (TT4) were measured by immunofluorescence. The morphological change of the myocardial tissue was observed by HIM staining. The expression of mir-124 and IGF-1 mRNA was determined by real-time quantitative PCR. The expression of IGF-1 protein was quantified by Western blot analysis. At week 2, the TSH level in hypothyroidism and L-T4 treatment groups was significantly higher compared with control group (P < 0.01), whereas the TT4 level in both groups was significantly lower (P < 0.01). At week 12, the TSH level in hypothyroidism group was significantly higher compared with L-T4 treatment and control group (P < 0.01), but the TT4 level in hypothyroidism group was significantly lower (P < 0.01). The mir-124 expression in hypothyroidism group was significantly higher compared with both L-T4 treatment and control group (P < 0.05). The expression of IGF-1 protein in hypothyroidism group was significantly lower compared with L-T4 treatment and control group (P < 0.05). The level of thyroid hormone was reduced in the thyroid gland in hypothyroid rats. IGF-1 expression was inhibited through increasing the myocardial mir-124 level.收起
