Objective:To investigate the neuroprotective effects of estradiol(E2)may be related to the interact with Grow Hormone(GH).Method:The protective effect of GH in different dose level(5,25,125,250,500 ng·mL-1)was observed in primary culture of rat hippocampal neurons for 24 hours through CCK-8 colorimetry.Phosphorylated-STAT5(P-STAT5)and STAT5 protein levels were tested in primary culture of rat hippocampal neurons after 24 h of serum starvation and treated for 60 min with different doses of GH(0.1...更多
Objective:To investigate the neuroprotective effects of estradiol(E2)may be related to the interact with Grow Hormone(GH).Method:The protective effect of GH in different dose level(5,25,125,250,500 ng·mL-1)was observed in primary culture of rat hippocampal neurons for 24 hours through CCK-8 colorimetry.Phosphorylated-STAT5(P-STAT5)and STAT5 protein levels were tested in primary culture of rat hippocampal neurons after 24 h of serum starvation and treated for 60 min with different doses of GH(0.1~20 ng·mL-1),or different time of GH(15~90 min)to observe GH the optimal concentration and action time.SOCS2 protein levels were tested in primary culture of rat hippocampal neurons after 24 h of serum starvation and treated for 60 min with different doses of E2(10-11~10-7 mol·L-1),or different time of E2(15~90 min)to observe E2 the optimal concentration and action time.P-STAT5 and STAT5 protein levels were tested in primary culture of rat hippocampal neurons after 24 h of serum starvation and treated for 60 min with GH(5 ng·mL-1),or E2(10-8 mol·L-1),or E2+GH.In this latter case E2 was added 60 min before GH.SOCS1,SOCS2 and SOCS3 mRNA and protein levels were detected in 24 h serum starved rat hippocampal neurons after 60 min treatment with E2(10-8 mol·L-1).SOCS2 mRNA and protein levels were detected in 24 h serum starved rat hippocampal neurons after 60 min treatment with GH(5 ng·mL-1),or E2(10-8 mol·L-1),or E2+GH.In this latter case E2 was added 60 min before GH.Result:It was testified that GH was nontoxic to rat hippocampal neurons in the range of 5~250 ng·mL-1.However,if GH concentration was over 250 ng·mL-1,the activity of hippocampal neurons began to decrease.STAT5 and P-STAT5 protein levels were response to increasing concentrations of GH(0.1~20 ng·mL-1)in rat hippocampal neurons after 60 min of treatment,which determined optimal concentration and effect time of GH.SOCS2 protein levels were response to increasing concentrations of E2(10-11~10-7 mol·L-1)in rat hippocampal neurons after 60 min of treatment,收起
