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作者

Hao Liu Lin Zhong Chunling Zhao Xiaolei Tang Jun Yang Lei Gong

作者单位

Yuhuangding Hospital, Yantai, Shandong Province, China. Weifang Medical College, Weifang, Shandong Province, China. Taishan Medical College, Taian, Shandong Province, China.

刊名

Cell biology international

年份

2018

ISSN

1095-8355

关键词

CDNF endoplasmic reticulum stress luciferase neurotrophic factor secretion

分类号

571.6

摘要

Cerebral dopamine neurotrophic factor has been considered as potent candidates for the therapy of Parkinson's disease for which can promote the survival of midbrain dopaminergic neurons. In addition to secret out from cells like other classical neurotrophic factors , CDNF can locate in the endoplasmatic reticulum , where they can function as ER stress response protein to regulate ER stress. In our previous studies, we have found two helices, α1 and α7, which can regulate the intracellular traf...更多

Cerebral dopamine neurotrophic factor has been considered as potent candidates for the therapy of Parkinson's disease for which can promote the survival of midbrain dopaminergic neurons. In addition to secret out from cells like other classical neurotrophic factors , CDNF can locate in the endoplasmatic reticulum , where they can function as ER stress response protein to regulate ER stress. In our previous studies, we have found two helices, α1 and α7, which can regulate the intracellular trafficking and secretion of CDNF. α1 distruction can significantly retain CDNF protein in the ER, but α7 distruction induce most CDNF protein secreting out the cells. Then α1 and α7 regulate protein trafficking and secretion in opposite side. However, the exact secretion level of CDNF affected by α1 or α7 have not been sensitively quantified. In this study, we used nanoluciferase to quantify the secretion level of CDNF protein so that we could evaluate the impact of α1 and α7 on CDNF secretion or function.收起

学科

【SCI学科】CELL BIOLOGY 【ESI学科】MOLECULAR BIOLOGY & GENETICS

影响因子

2016年:1.831;2015年:1.663;2014年:1.933;2013年:1.635;2012年:1.64

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期刊

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