Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Diseases, Taishan Medical College, Tai'an City, ChinaHainan Provincial Key Lab. of Tropical Animal Reproduction and Breeding and Veterinary Medicine, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, Chinaa Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, College of Veterinary Medicine, Shandong Agricultural University, Tai'an City, China
Mitochondrial antiviral-signaling protein , an adaptor protein of retinoic acid-inducible gene I -like receptors -mediated signal pathway, is involved in innate immunity. In this study, Cherry Valley duck MAVS was cloned from the spleen and analyzed. duMAVS was determined to have a caspase activation and recruitment domain at N-terminal, followed by a proline-rich domain and a transmembrane domain at C-terminal. Quantitative real-time PCR indicated that duMAVS was expressed in all tissues teste...更多
Mitochondrial antiviral-signaling protein , an adaptor protein of retinoic acid-inducible gene I -like receptors -mediated signal pathway, is involved in innate immunity. In this study, Cherry Valley duck MAVS was cloned from the spleen and analyzed. duMAVS was determined to have a caspase activation and recruitment domain at N-terminal, followed by a proline-rich domain and a transmembrane domain at C-terminal. Quantitative real-time PCR indicated that duMAVS was expressed in all tissues tested across a broad expression spectrum. The expression of duMAVS was significantly upregulated after infection with duck Tembusu virus . Overexpression of duMAVS could drive the activation of interferon -β, nuclear factor-κB, interferon regulatory factor 7, and many downstream factors in duck embryo fibroblast cells. What is more, RNA interference further confirmed that duMAVS was an important adaptor for IFN-β activation. The antiviral assay showed that duMAVS could suppress the various viral replications at early stages of infection. Overall, these results showed that the main signal pathway mediated by duMAVS and it had a broad-spectrum antiviral ability. This research will be helpful to better understanding the innate immune system of ducks.收起
