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作者

Shen, F. Walker, E.J. Jiang, L. Degos, V. Li, J. Sun, B. Heriyanto, F. Young, W.L. Su, H.

作者单位

Department of Neurology, Affiliated Hospital, Taishan Medical College, Taishan, China Department of Neurological Surgery, University of California, San Francisco, CA, United States a Department of Anesthesia and Perioperative Care, Center for Cerebrovascular Research, University of California, 1001 Potrero Avenue, San Francisco, CA 94110, United States Department of Neurology, University of California, San Francisco, CA, United States

刊名

Journal of Cerebral Blood Flow and Metabolism

年份

2011

卷号

Vol.31 No.12

页码

2343-2351

ISSN

0271-678X

关键词

adeno-associated viral vector angiopoietin-1 middle cerebral artery occlusion tight junction protein vascular integrity VEGF

分类号

616.8

摘要

Vascular endothelial growth factor -induced neovasculature is immature and leaky. We tested if coexpression of angiopoietin-1 with VEGF improves blood-brain barrier integrity and VEGF neuroprotective and neurorestorative effects using a permanent distal middle cerebral artery occlusion model. Adult CD-1 mice were injected with 2×10 9 virus genomes of adeno-associated viral vectors expressing VEGF or ANG1 individually or together in a 1:1 ratio into the ischemic penumbra 1 hour after pMCAO...更多

Vascular endothelial growth factor -induced neovasculature is immature and leaky. We tested if coexpression of angiopoietin-1 with VEGF improves blood-brain barrier integrity and VEGF neuroprotective and neurorestorative effects using a permanent distal middle cerebral artery occlusion model. Adult CD-1 mice were injected with 2×10 9 virus genomes of adeno-associated viral vectors expressing VEGF or ANG1 individually or together in a 1:1 ratio into the ischemic penumbra 1 hour after pMCAO. AAV-LacZ was used as vector control. Samples were collected 3 weeks later. Compared with AAV-LacZ, coinjection of AAV-VEGF and AAV-ANG1 reduced atrophy volume ; injection of AAV-VEGF or AAV-ANG1 individually reduced atrophy volume slightly . Overexpression of VEGF reduced tight junction protein expression and increased Evans blue extravasation. Compared with VEGF expression alone, coexpression of ANG1 with VEGF resulted in upregulation of tight junction protein expression and reduction of Evans blue leakage . Coinjection of AAV-VEGF and AAV-ANG1 induced a similar degree of angiogenesis as injection of AAV-VEGF alone . Thus, coexpression of ANG1 with VEGF improved BBB integrity and resulted in better neuroprotection.收起

学科

【SCI学科】ENDOCRINOLOGY & METABOLISM;NEUROSCIENCES;HEMATOLOGY 【ESI学科】NEUROSCIENCE & BEHAVIOR

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