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作者

Gao, LL Li, FR Si, YH Qin, SC

作者单位

Taishan Med Univ, Atherosclerosis Inst, Dept Pathol & Pathophysiol, Tai An, Shandong, Peoples R China

刊名

FUTURE MATERIALS ENGINEERING AND INDUSTRY APPLICATION

年份

2012

卷号

Vol.365

页码

348-353

ISSN

1022-6680

关键词

Paris Polyphylla Smith extracts oxidation stress apoptosis

摘要

Paris Polyphylla Smith is widely used in traditional Chinese medicine. For its effective of antipyretic detoxicate, its active component is steroid saponin([1]). The present study was designed to evaluate the antioxidative effect of extracts of Paris Polyphylla Smith(PPSE) and its capacity to protect human umbilical vein endothelial cells (HUVECs) against oxidative damage induced by oxidized low density lipoprotein (ox-LDL). Cytotoxicity was determined by MTT assay. Flow cytometer (FCM) demonstr...更多

Paris Polyphylla Smith is widely used in traditional Chinese medicine. For its effective of antipyretic detoxicate, its active component is steroid saponin([1]). The present study was designed to evaluate the antioxidative effect of extracts of Paris Polyphylla Smith(PPSE) and its capacity to protect human umbilical vein endothelial cells (HUVECs) against oxidative damage induced by oxidized low density lipoprotein (ox-LDL). Cytotoxicity was determined by MTT assay. Flow cytometer (FCM) demonstrated that the increased apoptosis induced by oxidized low density were inhibited, and the blocked transition of cells from static phase (G0/G1) to DNA synthesis (S) were restored by PPSE, these effect were all in a dose-dependent manner. The actions by PPSE to reduce apoptosis may help protect endothelial function and inhibit atherogenesis, while the novel actions to mitigates oxidative stress may weaken the ability of endothelial injury. Further studies are needed to identify the chemical compounds responsible in order to develop its clinical application.收起

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