获取途径

作者

Liu, Yong Qi, Bing Xie, Juan Wu, Xiaoqing Ling, Yinghui Cao, Xinyan Kong, Feng Xin, Jing Jiang, Xin Wu, Qiaoqin

作者单位

Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Lab Anim Genet Resources Protect & Bre, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China Taishan Med Univ, Sch Life Sci, Tai An 271016, Shandong, Peoples R China Fuyang Normal Univ, Key Lab Embryo Dev Reprod Regulat Anhui Prov, Fuyang 236041, Anhui, Peoples R China Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, 4899 Juye St, Changchun 130112, Jilin, Peoples R China

刊名

BMC GENOMICS

年份

2018

卷号

Vol.19

ISSN

1471-2164

关键词

LNCRNA IDENTIFICATION TRANSCRIPTION GENE ANNOTATION MATURATION PATHWAY CELLS LYASE KEGG

摘要

BackgroundThe goat is an important farm animal. Reproduction is an important process of goat farming. The ovary is the most important reproductive organ for goats. In recent years, an increasing number of long non-coding RNAs have been implicated in the regulation of mammal reproduction. However, there are few studies on the function of lncRNAs in reproduction, particularly lncRNAs in the ovary.ResultsThe sequencing of goat ovaries generated 1,122,014,112 clean reads, and 4926 lncRNAs and 1454 ...更多

BackgroundThe goat is an important farm animal. Reproduction is an important process of goat farming. The ovary is the most important reproductive organ for goats. In recent years, an increasing number of long non-coding RNAs have been implicated in the regulation of mammal reproduction. However, there are few studies on the function of lncRNAs in reproduction, particularly lncRNAs in the ovary.ResultsThe sequencing of goat ovaries generated 1,122,014,112 clean reads, and 4926 lncRNAs and 1454 TUCPs were identified for further analysis by using the coding potential analysis software, CNCI, CPC and Pfam-sca. There were 115 /22 differential lncRNAs /TUCPs transcripts between the ovaries of the luteal phase and the follicular phase. We predicted the related genes of lncRNA /TUCP based on co-expression and co-localization methods. In total, 2584 /904 genes were predicted by co-expression, and 326/73 genes were predicted by co-localization. The functions of these genes were further analyzed with GO and KEGG analysis. The results showed that lncRNAs /TUCPs, which are highly expressed in goat ovaries in the luteal phase, are mainly associated with the synthesis of progesterone, and we filtered the lncRNAs /TUCPs, such as XR_001918177.1 and TUCP_001362, which may regulate the synthesis of progesterone; lncRNAs /TUCPs, which are highly expressed in goat ovaries in the follicular phase, are mainly associated with oogenesis and the maturation of oocytes, and we filtered the lncRNAs /TUCPs that may regulate the oogenesis and maturation of oocyte, such as XR_001917388.1 and TUCP_000849.ConclusionThe present study provided the genome expression profile of lncRNAs /TUCPs in goat ovaries at different estrus periods and filtered the potential lncRNAs /TUCPs associated with goat reproduction. These results are helpful to further study the molecular mechanisms of goat reproduction.收起

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