Department of Cardiology, the 88th Hospital of the Chinese PLA, Taian, 271000 ChinaDepartment of Critical Care Medicine, the Chinese PLA General Hospital, Beijing, 100853 ChinaDepartment of Orthopedics, the 148th Hospital of the Chinese PLA, Zibo, 255300 ChinaDepartment of Respiratory Disease, the 88th Hospital of the Chinese PLA, Taian, 271000 ChinaDepartment of Medical Services, the 88th Hospital of the Chinese PLA, Taian, 271000 ChinaDepartment of TCM, The Affiliated Hospital of Taishan Medical College, Taian, 271000 China
刊名
Experimental Physiology
年份
2015
卷号
Vol.100 No.3
页码
331-340
ISSN
1469-445X
摘要
New Findings What is the central question of this study? It is not known whether treatment with interleukin‐10 attenuates hyperoxia‐induced acute lung injury in mice. What is the main finding and its importance? Our results showed that exogenous IL‐10 treatment alleviated hyperoxia‐induced acute lung injury in mice, possibly by regulating neutrophil recruitment and the subsequent generation of cytokines, nitric oxide and matrix metalloproteinases. Lung injury caused by breathing air en...更多
New Findings What is the central question of this study? It is not known whether treatment with interleukin‐10 attenuates hyperoxia‐induced acute lung injury in mice. What is the main finding and its importance? Our results showed that exogenous IL‐10 treatment alleviated hyperoxia‐induced acute lung injury in mice, possibly by regulating neutrophil recruitment and the subsequent generation of cytokines, nitric oxide and matrix metalloproteinases. Lung injury caused by breathing air enriched with oxygen continues to be a major problem in clinical medicine. Here, we investigated the therapeutic role of interleukin‐10 in hyperoxia‐induced acute lung injury in mice. In the first experiment, mice were exposed to room air or 95% O 2 and treated with IL‐10 simultaneously. In the second experiment, wild‐type mice and IL‐10 −/− mice were exposed to room air or 95% O 2 . Exogenous IL‐10 treatment attenuated hyperoxia‐induced acute lung injury, evidenced by a reduced ratio of lung weight to body weight, ratio of lung wet weight to dry weight, cell numbers and protein content in bronchoalveolar lavage fluid and cell death. Interleukin‐10 treatment markedly prolonged the survival of mice during oxygen exposure. Interleukin‐10 treatment reduced the activity of myeloperoxidase and mRNA levels of interleukin‐6, tumour necrosis factor‐α and macrophage inflammatory protein 2, suppressed nuclear factor‐κB activation and decreased inducible nitric oxide synthnase expression and nitric oxide formation in lungs of mice exposed to hyperoxia. Interleukin‐10 treatment suppressed activities of matrix metalloproteinase 2 and matrix metalloproteinase 9 and reduced lung permeability in mice during oxygen exposure. Furthermore, absence of IL‐10 aggravated hyperoxia‐induced acute lung injury and reduced the duration of survival of mice during oxygen exposure, which was attenuated by treatment with IL‐10. In conclusion, our results show that exogenous IL‐10 treatment alleviates hyperoxia‐induced acute lung injury in mice, possibly by regulating neutrophil recruitment and the subsequent generation of cytokines, nitric oxide and matrix metalloproteinases. This suggests that IL‐10 treatment may be a promising therapeutic strategy to reduce lung injury in patients exposed to hyperoxia.收起
