Department of Neurology, Hospital of Taishan Medical College, Taian 271000 Shandong Province, China Department of Neurology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022 Hubei Province, China
刊名
Chinese Journal of Clinical Rehabilitation
年份
2006
卷号
Vol.10 No.11
页码
59-61
ISSN
1671-5926
摘要
Aim: To investigate the effect of astragalus polysaccharides characterized by anti-inflammation, adjusting immune and delaying neuronal degeneration on contents of estathion and malondialdehyde , activities of glutathione peroxidase and superoxide dismutase in astrocyte culture medium, and to discuss protective effect of APS on dynamic equilibrium injury of free radical system in bendopa-induced astrocyte culture medium so as to analyze the relationship with time. Methods: The experiment was c...更多
Aim: To investigate the effect of astragalus polysaccharides characterized by anti-inflammation, adjusting immune and delaying neuronal degeneration on contents of estathion and malondialdehyde , activities of glutathione peroxidase and superoxide dismutase in astrocyte culture medium, and to discuss protective effect of APS on dynamic equilibrium injury of free radical system in bendopa-induced astrocyte culture medium so as to analyze the relationship with time. Methods: The experiment was completed in the Basic Research Institute of Taishan Medical College from December 2003 to September 2004. 1 Ten postnatal newborn SD rats, with 2-day old, of either gender, were selected, 2 Primary culture of astrocyte was performed with McCarthy and deVelli methods, and astrocyte culture medium was used to establish injured model of bendopa. Rats were divided into 3 groups : normal control group , bendopa group and APS + bendopa group [20 mg/L APS and 100 μmol/Lbendopa]. Culture medium was dealt with after 4, 24, 48 and 72 hours after culture respectively. 3 Content of estathion was assayed with colorimetric quantity technique; activity of glutathione peroxidase with chromatometry; SOD activity with xanthine oxidase method; and MDA content with thiolbarbituric acid method. 4 Measurement data were compared with analysis of variance. Results: 1 Content of estathion and activities of glutathione peroxidase and SOD: There was not significant difference after 4-hour culture. Values in bendopa group were lower than those in blank control group 24, 48 and 72 hours after culture , but were higher than those in bendopa group , but were lower than those in bendopa group . Conclusion: Free radical produced by self-oxidation of bendopa can promote development of Parkinson disease. APS can relieve the toxicity of bendopa on neurons and the effect has time dependency.收起
