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作者

Gao, Y. Guo, Q. Li, M. Jia, F. Yan, K. Zheng, C.

作者单位

a College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China College of Life Sciences, Taishan Medical University, Tai'an, 271016, China

刊名

Chinese Journal of Applied and Environmental Biology

年份

2017

卷号

Vol.23 No.2

页码

220-224

ISSN

1006-687X

关键词

Heat shock factor Seed-specific promoter Thermotolerance Transgenic Nicotiana tabacum

摘要

Gene expression is regulated at multiple levels, and a promoter plays a crucial role in determining the temporal and spatial expression patterns and transcriptional levels of genes. Under the regulation of tissue-specific promoters, genes express in a particular space and time, which can avoid unnecessary energy losses in unexpected organs. This property can be used to improve crop quality and adaptability for surviving under adverse conditions. In this study, tissue-specific promoters were used...更多

Gene expression is regulated at multiple levels, and a promoter plays a crucial role in determining the temporal and spatial expression patterns and transcriptional levels of genes. Under the regulation of tissue-specific promoters, genes express in a particular space and time, which can avoid unnecessary energy losses in unexpected organs. This property can be used to improve crop quality and adaptability for surviving under adverse conditions. In this study, tissue-specific promoters were used to drive the expression of resistance genes. First, Arabidopsis seed-specific promoter At2S3 and heat shock transcription factor AtHsfA6a were cloned i nto pBI121 binary vector . Next, this vector was transformed into tobacco, and stable transgenic tobacco lines were screened. Finally, seed germination experiment was performed to determine the effects on heat stress. The results indicated that the expression of AtHsfA6a increased remarkably under temperature stress. Its expression could be upregulated 28 times after heat treatment at 45 °C. After 12, 18, and 24 h of the heat treatment, the germination rates of transgenic tobaccos were 100% and 98%; 98% and 94%; and 97% and 92%, respectively, whereas those of wild-type plants were just 80%, 76%, and 70%, respectively. Transgenic plants were found to have acquired thermotolerance at the root elongation stage after heat treatment. These results suggested that the transgenic lines are more tolerant to heat stress than wild-type plants. This study provides direct experimental evidence that the combination of seed-specific promoters and resistant genes can be used to improve plant tolerance against adversity stress, which could be used as a new strategy for genetic engineering breeding.收起

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