获取途径

作者

Ren, Hao Yang, Xiaomin Yang, Yongmei Zhang, Xiaoyu Zhao, Rui Wei, Ran Zhang, Xin Zhang, Yi

作者单位

Taishan Med Univ, Clin Med Undergrad, Tai An, Shandong, Peoples R China Qingdao Univ, Affiliated Hosp, Dept Clin Lab, Qingdao, Shandong, Peoples R China Wakayama Med Univ, Wakayama, Japan Qingdao Univ, Yuhuangding Hosp, Med Coll, Dept Lab, Yantai, Shandong, Peoples R China Shandong Univ, Qilu Hosp, Dept Clin Lab, Jinan, Shandong, Peoples R China

刊名

ONCOTARGET

年份

2018

卷号

Vol.9 No.4

页码

4851-4861

ISSN

1949-2553

关键词

BCYRN1 lncRNA gastric carcinoma tumor progression EpCAM

分类号

616.992

摘要

Brain cytoplasmic RNA 1 , along non-coding RNA, plays a critical role in various diseases, including some cancers. However, the expression of BCYRN1 and its roles in gastric carcinoma still remain unidentified. Thus, this study employed RT-qPCR to detect expression of BCYRN1 in 85 paired GC samples and adjacent normal tissues, and performed in vitro studies to explore effects of BCYRN1 in GC cells on cell proliferation, apoptosis and migration. We found BCYRN1 was significantly upregulated in G...更多

Brain cytoplasmic RNA 1 , along non-coding RNA, plays a critical role in various diseases, including some cancers. However, the expression of BCYRN1 and its roles in gastric carcinoma still remain unidentified. Thus, this study employed RT-qPCR to detect expression of BCYRN1 in 85 paired GC samples and adjacent normal tissues, and performed in vitro studies to explore effects of BCYRN1 in GC cells on cell proliferation, apoptosis and migration. We found BCYRN1 was significantly upregulated in GC samples, and its expression was positively correlated with advanced TNM stage and tumor size . Functionally, BCYRN1 knockdown by siRNA could inhibit cell proliferation, induce G1/G0 cell cycle arrest, increase apoptosis and impair migratory ability of AGS cells. Moreover, the results of RT-qPCR and western blotting indicated that knockdown of BCYRN1 notably decreased the expression of epithelial cell adhesion molecules . Otherwise, overexpression of BCYRN1 in GC cells could reverse the effects of BCYRN1 knockdown. Taken together, our data indicate for the first time that BCYRN1 acts as an oncogenic lncRNA in GC progression and may be a potential therapeutic target in GC.收起

学科

【SCI学科】CELL BIOLOGY;ONCOLOGY 【ESI学科】MOLECULAR BIOLOGY & GENETICS

影响因子

2016年:5.168;2015年:5.008;2014年:6.359;2013年:6.627;2012年:6.636

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期刊

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