Expression of a novel thermostable Cu, Zn-superoxide dismutase from Chaetomium thermophilum in Pichia pastoris and its antioxidant properties

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作者单位: Taishan Med Coll, Dept Chem & Chem Engn, Tai An 271016, Shandong, Peoples R China Taishan Medical University Shandong Agr Univ, Dept Environm Biol, Tai An 271018, Shandong, Peoples R China Shandong Agricultural University

摘要: A new superoxide dismutase gene from the thermophilic fungus Chaetomium thermophilum was cloned and expressed in Pichia pastoris and its gene product was characterized. The specific activity of the purified CtSOD was 2,170 U/mg protein. The enzyme was inactivated by KCN and HO but not by NaN, confirming that it belonged to the type of Cu, ZnSOD. The amino acid residues involved in coordinating copper and zinc were conserved. The recombinant CtSOD exhibited optimum activity at pH 6.5 and 60 deg...更多; A new superoxide dismutase gene from the thermophilic fungus Chaetomium thermophilum was cloned and expressed in Pichia pastoris and its gene product was characterized. The specific activity of the purified CtSOD was 2,170 U/mg protein. The enzyme was inactivated by KCN and HO but not by NaN, confirming that it belonged to the type of Cu, ZnSOD. The amino acid residues involved in coordinating copper and zinc were conserved. The recombinant CtSOD exhibited optimum activity at pH 6.5 and 60 degrees C. The enzyme retained 65% of the maximum activity at 70 degrees C for 60 min and the half-life was 22 and 7 min at 80 and 90 degrees C, respectively. The recombinant yeast exhibited higher stress resistance than the control yeast cells to salt and superoxide-generating agents, such as paraquat and menadione.收起

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