The Combinations Chitosan-PamCSK and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc

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作者: Yongli Bi Qingan Xu Lingkai Su Jiantao Xu Zhongfang Liu Ying Yang Hua Tang Yuhong Li Mingwen Fan

作者单位: Georgia Institute of Technology School of Biological Sciences The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China Institute of Immunology, Taishan Medical University, Taian, China Department of Endodontics and Key Laboratory for Oral Biomedical Research of Zhejiang Province, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, China School of Medicine, Jianghan University, Wuhan, ChinaWuhan First Stomatological Hospital, Wuhan, China Taikang Tongji (Wuhan) Hospital, Wuhan, China

摘要: We previously demonstrated that recombinant protein PAc could be administered as an anticaries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan plus Pam 3 CSK 4 and of chitosan plus monophosphoryl lipid A in the immune responses to the PAc protein in vivo and in vitro. PAc-chitosan-Pam 3 CSK 4 or PAc-chitosan-MPL promoted significantly higher PAc-specific antibody tite...更多; We previously demonstrated that recombinant protein PAc could be administered as an anticaries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan plus Pam 3 CSK 4 and of chitosan plus monophosphoryl lipid A in the immune responses to the PAc protein in vivo and in vitro. PAc-chitosan-Pam 3 CSK 4 or PAc-chitosan-MPL promoted significantly higher PAc-specific antibody titers in serum and saliva, inhibited Streptococcus mutans colonization onto the tooth surfaces, and endowed better protection effect with significantly less caries activities than PAc alone. Chitosan-Pam 3 CSK 4 and chitosan-MPL showed no statistically significant differences. In conclusion, our study demonstrated that the chitosan-Pam 3 CSK 4 and chitosan-MPL combinations are promising for anticaries vaccine development.收起

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