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作者

Fei, H.R. Chen, H.L. Xiao, T. Chen, G. Wang, F.Z.

作者单位

Department of Biology, Taishan Medical University, Chang Cheng Road, Taian 271016, China a School of Pharmacy, Taishan Medical University, Chang Cheng Road, Taian 271016, China

刊名

Molecular Biology Reports

年份

2012

卷号

Vol.39 No.1

页码

131-138

ISSN

0301-4851

关键词

Bcl-2 Caspase Caudatin Cell apoptosis Hepatoma cell MAPK

摘要

In the present study, we investigate the anti-cancer activity and mechanism of caudatin, the C-21 steroidal glycosides, on human hepatoma cell line HepG2. The MTT assay and flow cytometry were used to evaluate HepG2 cell proliferation and cell cycle. Annexin-V/PI and DAPI staining were used to investigate cell apoptosis. Western blotting analysis was used to evaluate the expression levels of proteins. It is found that caudatin inhibits HepG2 cell growth and induces of G 0 /G 1 phase arrest in...更多

In the present study, we investigate the anti-cancer activity and mechanism of caudatin, the C-21 steroidal glycosides, on human hepatoma cell line HepG2. The MTT assay and flow cytometry were used to evaluate HepG2 cell proliferation and cell cycle. Annexin-V/PI and DAPI staining were used to investigate cell apoptosis. Western blotting analysis was used to evaluate the expression levels of proteins. It is found that caudatin inhibits HepG2 cell growth and induces of G 0 /G 1 phase arrest in a dose dependent manner, which is associated with a decreased in the expression of cyclinD 1 and increased the levels of p21 and p53. HepG2 cells dealing with caudatin showed typical characteristics of apoptosis. Western blotting analysis indicated that the levels of Bcl-2 were down-regulated after caudatin treatment, whereas the expression of Bax was up-regulated. Furthermore, caudatin-induced apoptosis was accompanied by activation of caspase-3, -9, and poly Polymerase . Treatment with caudatin also induced phosphorylation of extracellular-signal regulating kinase and c-Jun N-terminal kinase . These results demonstrate that caudatin inhibits cell proliferation via DNA synthesis reduction and induces caspase-dependent apoptosis in HepG2 cell. Activation of ERK and JNK may be involved in caudatin-induced hepatoma cell apoptosis.收起

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