获取途径

作者

Lv B. Zhang L. Miao R. Xiang X. Dong S. Lin T. Li K. Kai Q.

作者单位

Department of Blood Transfusion, Liaocheng People's Hospital, Taishan Medical College, Liaocheng, Shandong Province, China Department of Pediatric Surgery, Liaocheng People's Hospital, Taishan Medical College, Liaocheng, Shandong Province, China Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China Department of Surgical Intensive Care Units, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China Department of Central Laboratory, Liaocheng People's Hospital, Liaocheng, China

刊名

Biomedicine and Pharmacotherapy

年份

2018

卷号

Vol.98

页码

783-792

ISSN

0753-3322

关键词

Hepatoblastoma Hippo-YAP signaling pathway LINC01314 lncRNA Prognosis

摘要

Hepatoblastoma , as a common pediatric liver malignancy, is composed of a variety of subgroups with different clinical outcomes. Long-noncoding RNA has crucial roles in cancer biology. However, the association between lncRNA and HB has not been fully investigated. In this study, we screened lncRNA expression profiles that were annotated from the GSE75271 dataset. A total of 225 differentially expressed lncRNAs were identified based on comparison between three prognostic subgroups, and seven of...更多

Hepatoblastoma , as a common pediatric liver malignancy, is composed of a variety of subgroups with different clinical outcomes. Long-noncoding RNA has crucial roles in cancer biology. However, the association between lncRNA and HB has not been fully investigated. In this study, we screened lncRNA expression profiles that were annotated from the GSE75271 dataset. A total of 225 differentially expressed lncRNAs were identified based on comparison between three prognostic subgroups, and seven of them that exhibited highly predictive accuracies were selected for functional analysis. Weighted gene correlation network analysis was employed to predict the biological functions of the seven DELs. The Hippo-YAP signaling pathway was predicted to be the most statistically significant predicted pathway associated with the seven DELs. Furthermore, we performed in vitro experiments to validate the biological function of one DEL, NR_120317 . Our results showed decreased proliferation and migration activities of HB cells overexpressing LINC01314. Moreover, mechanistic investigations revealed that LINC01314 overexpression inhibited nuclear translocation of YAP, by inducing MST1 expression and promoting phosphorylation of LATS1 and YAP, consequently downregulating the expression of cell cycle regulatory proteins . Taken together, our findings provide evidence for LINC01314 as a potential biomarker and anti-cancer therapeutic target in patients with HB.收起

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