Jinan Central Hospital Affiliated to Shandong University, Jinan, China.People's Hospital of Liaocheng Affiliated to Taishan Medical College, Taishan, China.Qilu Hospital of Shandong University, Jinan, China.Affiliations
This study aimed to identify the gene expression changes conferred by capsaicin in the cell model of 6-OHDA-induced Parkinson's disease, to disclosure the molecular mechanism of action of capsaicin. We used capsaicin-treated and paraffin-embedded wax blocks containing substantia nigra tissue from 6-OHDA-induced Parkinson's disease rats to analyze transcriptional changes using Affymetrix GeneChip Whole Transcript Expression Arrays. A total of 108 genes were differentially expressed in response to...更多
This study aimed to identify the gene expression changes conferred by capsaicin in the cell model of 6-OHDA-induced Parkinson's disease, to disclosure the molecular mechanism of action of capsaicin. We used capsaicin-treated and paraffin-embedded wax blocks containing substantia nigra tissue from 6-OHDA-induced Parkinson's disease rats to analyze transcriptional changes using Affymetrix GeneChip Whole Transcript Expression Arrays. A total of 108 genes were differentially expressed in response to capsaicin treatment, and 7 of these genes were selected for further analysis: Olr724, COX1, Gsta2, Rab5a, Potef, Actg1, and Acadsb, of which Actg1 was downregulated and Gsta2 was upregulated. We successfully overexpressed Actg1 and Gsta2 in vitro. CCK-8 detection and flow cytometry demonstrated that overexpression of Actg1 and Gsta2 increased apoptosis in the 6-OHDA-induced Parkinson's disease cell model. The imbalance between Actg1 and Gsta2 may be one of the mechanisms of cell damage in Parkinson's disease . Capsaicin can protect the cells and reduce the apoptosis rate by regulating Actg1 and Gsta2.收起
发文期刊《Regulation of Actg1 and Gsta2 Is Possible Mechanism by Which Capsaicin Alleviates Apoptosis in Cell Model of 6-OHDA-induced Parkinson's Disease》历年引证文献趋势图
