Department of Joint Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong Province, China Department of Orthopaedics, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong Province, China Central Laboratory, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical College, Liaocheng, Shandong Province, China Department of Pathology, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong Province, China
刊名
Brazilian Journal of Medical and Biological Research
Transforming growth factor beta 1 and bone morphogenetic protein-2 are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractu...更多
Transforming growth factor beta 1 and bone morphogenetic protein-2 are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractured tibias were measured by immunohistochemistry and quantitative reverse-transcription polymerase chain reaction, weekly for the first 5 weeks post-fracture. Mechanical parameters of the healing bones were also assessed at 3, 4, and 5 weeks post-fracture, after the rats were sacrificed. The bending rigidity, torsional rigidity and destruction torque of the two groups increased continuously during the healing process. The diabetes group had lower mean values for bending rigidity, torsional rigidity and destruction torque compared with the control group . TGF-β1 and BMP-2 expression were significantly lower in the control group than in the diabetes group at postoperative weeks 1, 2, and 3. Peak levels of TGF-β1 and BMP-2 expression were delayed by 1 week in the diabetes group compared with the control group. Our results demonstrate that there was a delayed recovery in the biomechanical function of the fractured bones in diabetic rats. This delay may be associated with a delayed expression of the growth factors TGF-β1 and BMP-2.收起
发文期刊《Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing》历年引证文献趋势图
