获取途径

作者

M.T. Xu S. Sun L. Zhang F. Xu S.L. Du X.D. Zhang D.W. Wang

作者单位

Department of Joint Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong Province, China Department of Orthopaedics, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong Province, China Central Laboratory, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical College, Liaocheng, Shandong Province, China Department of Pathology, Liaocheng People’s Hospital, Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong Province, China

刊名

Brazilian Journal of Medical and Biological Research

年份

2016

卷号

Vol.49 No.1

ISSN

0100-879x;1414-431x

关键词

Biomechanical function BMP-2 Diabetes mellitus Fracture healing Mechanical parameters TGF-beta1

摘要

Transforming growth factor beta 1 and bone morphogenetic protein-2 are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractu...更多

Transforming growth factor beta 1 and bone morphogenetic protein-2 are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractured tibias were measured by immunohistochemistry and quantitative reverse-transcription polymerase chain reaction, weekly for the first 5 weeks post-fracture. Mechanical parameters of the healing bones were also assessed at 3, 4, and 5 weeks post-fracture, after the rats were sacrificed. The bending rigidity, torsional rigidity and destruction torque of the two groups increased continuously during the healing process. The diabetes group had lower mean values for bending rigidity, torsional rigidity and destruction torque compared with the control group . TGF-β1 and BMP-2 expression were significantly lower in the control group than in the diabetes group at postoperative weeks 1, 2, and 3. Peak levels of TGF-β1 and BMP-2 expression were delayed by 1 week in the diabetes group compared with the control group. Our results demonstrate that there was a delayed recovery in the biomechanical function of the fractured bones in diabetic rats. This delay may be associated with a delayed expression of the growth factors TGF-β1 and BMP-2.收起

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