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作者

Tingting Wang, Lin Zhu, Hongling Liu, Guoyi Yu, Yunliang Guo

作者单位

Editoral Office of Journal of Qingdao University (Medical Science), Qingdao, Shandong, China. Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao, Shandong, China. Affiliations Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

刊名

Anatomical record (Hoboken, N.J. : 2007)

年份

2019

卷号

Vol.302 No.12

页码

2245-2254

ISSN

1932-8494

关键词

JNK apoptosis autophagy oxygen glucose deprivation/reoxygen picroside II.

摘要

To explore the neuroprotective effect of picroside II on C-Jun NH2-terminal kinase signal pathway after oxygen glucose deprivation/reoxygen in SH-SY5Y cells. In vitro, SH-SY5Y cells were used to establish the OGD/R model, which was divided into the control group, model group, Picr group, and SP600125 group. Cellular viability was measured by CCK8. Cytotoxicity was assessed with LDH assay kit. Ad-GFP-mRFP-LC3 was used to monitor autophagosome and autolysosome. Apoptoic cells were detected by ...更多

To explore the neuroprotective effect of picroside II on C-Jun NH2-terminal kinase signal pathway after oxygen glucose deprivation/reoxygen in SH-SY5Y cells. In vitro, SH-SY5Y cells were used to establish the OGD/R model, which was divided into the control group, model group, Picr group, and SP600125 group. Cellular viability was measured by CCK8. Cytotoxicity was assessed with LDH assay kit. Ad-GFP-mRFP-LC3 was used to monitor autophagosome and autolysosome. Apoptoic cells were detected by Annexin V-FITC/PI apoptosis detection kit. The expressions of phospho-JNK and phospho-c-Jun were determined by western blot and immunofluorescence. The expressions of phospho-MKK4, phospho-Bcl-2, Bax, Beclin-1, and LC3 I/II were determined by WB. In the control group, only limited apoptosis and autophagy was observed, and the expression of associated proteins was very low. After OGD/R, the cellular viability of SH-SY5Y cells was reduced, whereas the cytotoxicity, apoptosis, and autophagy were increased, accompanied with an increase of phospho-MKK4, phospho-JNK, phospho-c-Jun, phospho-Bcl-2, LC3 II, Beclin-1, and Bax. During the reoxygen, treatment with Picr II or SP600125 could strengthen the cellular viability of SH-SY5Y cells, but repress the cytotoxicity, apoptosis, autophagy, and the expressions of associated protein. OGD/R could induce apoptosis and autophagy of SH-SY5Y cells by activating JNK signal pathway. Picr II could protect SH-SY5Y cells from autophagy and apoptosis following OGD/R by inhibiting JNK signal pathway. Anat Rec, 302:2245-2254, 2019.收起

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