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作者

Yulin Gao Jinjin Zhang Yuxia Liu Songzi Zhang

作者单位

Yuxia Liu ( School of Pharmaceutical Sciences, Binzhou Medical University, Department of Cellular and Genetic Medicine, Yantai City, China) Jinjin Zhang ( School of Pharmaceutical Sciences, Binzhou Medical University, Department of Cellular and Genetic Medicine, Yantai City, China) Yulin Gao ( School of Pharmaceutical Sciences, Binzhou Medical University, Department of Cellular and Genetic Medicine, Yantai City, China) Songzi Zhang ( Taishan Medical University, School of Pharmaceutical Sciences, Taian, China)

刊名

BMC Pulmonary Medicine

年份

2017

卷号

Vol.17 No.1

页码

1-15

ISSN

1471-2466

关键词

IPF lncRNA Telomere Mitochondria TERRA

摘要

Background Aging is a known risk factor of idiopathic pulmonary fibrosis . However, the pathogenic mechanisms underlying the effects of advanced aging remain largely unknown. Telomeric repeat-containing RNA represents a type of long noncoding RNA. In this study, the regulatory roles of TERRA on human telomeres and mitochondria and IPF epithelial injury model were identified. Methods Blood samples were collected from patients with IPF and matched control individuals . The significance of clinic...更多

Background Aging is a known risk factor of idiopathic pulmonary fibrosis . However, the pathogenic mechanisms underlying the effects of advanced aging remain largely unknown. Telomeric repeat-containing RNA represents a type of long noncoding RNA. In this study, the regulatory roles of TERRA on human telomeres and mitochondria and IPF epithelial injury model were identified. Methods Blood samples were collected from patients with IPF and matched control individuals . The significance of clinical research on the TERRA expression correlated with pulmonary fibrosis was assessed. The expression levels of TERRA in vivo and in vitro were determined through quantitative real-time polymerase chain reaction analysis. Telomerase activity was observed using a fluorescent quantitative TRAP assay kit. The functions of telomeres, mitochondria, and associated genes were analyzed through RNA interference on TERRA. Results TERRA expression levels significantly increased in the peripheral blood mononuclear cells of IPF patients. The expression levels also exhibited a direct and significantly inverse correlation with the percentage of predicted force vital capacity, which is a physiological indicator of fibrogenesis during IPF progression. This finding was confirmed in the epithelial injury model of IPF in vitro. RNA interference on TERRA expression can ameliorate the functions of telomeres; mitochondria; associated genes; components associated with telomeres, such as telomerase reverse transcriptase, telomerase, and cell nuclear antigen, cyclin D1; and mitochondria-associated cyclin E genes, including the MMP and Bcl-2 family. The RNA interference on TERRA expression can also improve the functions of oxidative-stress-associated genes, such as reactive oxygen species, superoxide dismutase, and catalase, and apoptosis-related genes, such as cytochrome c, caspase-9, and caspase-3. Conclusions In this study, the regulation of TERRA expression on telomeres and mitochondria during IPF pathogenesis was identified for the first time. The results may provide valuable insights for the discovery of a novel biomarker or therapeutic approach for IPF treatment.收起

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