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作者

Gao, Lidong Zou, Gang Liao, Qiaohong Zhou, Yonghong Liu, Fengfeng Dai, Bingbing Liu, Jia Chen, Zhiyong Xing, Weijia Yang, Le

作者单位

Hunan Provincial Center for Disease Control and Prevention, Changsha, China Anhua County Center for Disease Control and Prevention, Hunan Province, China Division of Infectious Disease, Key Laboratory of Surveillance and Early Warning on Infectious Disease, Chinese Centre for Disease Control and Prevention, Beijing, China School of Public Health, Fudan University, Key Laboratory of Public Health Safety, Ministry of Education, Shanghai, China School of Public Health, Fudan University, Key Laboratory of Public Health Safety, Ministry of Education, Shanghai, China Anhua People's Hospital, Hunan Province, China Division of Infectious Disease, Key Laboratory of Surveillance and Early Warning on Infectious Disease, Chinese Centre for Disease Control and Prevention, Beijing, China School of Public Health, Taishan Medical College, Taian, Shandong Province, China Division of Infectious Disease, Key Laboratory of Surveillance and Early Warning on Infectious Disease, Chinese Centre for Disease Control and Prevention, Beijing, China Institut Pasteur of Shanghai, Chinese Academy of Sciences, Beijing, China

刊名

Clinical Infectious Diseases

年份

2018

卷号

Vol.67 No.11

页码

1729-1735

ISSN

1058-4838

关键词

diagnostic yield enterovirus HFMD virological surveillance

摘要

Background Hand, foot, and mouth disease represents a substantial disease burden in the Western Pacific region. We investigated the spectrum of causative enteroviruses of HFMD, and evaluated different clinical samples' diagnostic yield for enteroviruses. Methods We enrolled pediatric patients hospitalized for HFMD among 6 hospitals in Anhua County, Hunan Province, China between October 2013 and September 2016. Throat swabs and stool samples were collected to detect the enterovirus serotypes by...更多

Background Hand, foot, and mouth disease represents a substantial disease burden in the Western Pacific region. We investigated the spectrum of causative enteroviruses of HFMD, and evaluated different clinical samples' diagnostic yield for enteroviruses. Methods We enrolled pediatric patients hospitalized for HFMD among 6 hospitals in Anhua County, Hunan Province, China between October 2013 and September 2016. Throat swabs and stool samples were collected to detect the enterovirus serotypes by real-time reverse-transcription polymerase chain reaction or nested PCR. Results Among the 2836 patients, only 1 developed severe illness. Seventeen serotypes were identified in 2401 patients , with the most frequently detected being CV-A16 , CV-A6 , EV-A71 , CV-A10 , and CV-A4 . Children were younger in CV-A6, CV-A10, and CV-A4 infections than EV-A71 and CV-A16 infections . The predominant enterovirus serotype shifted between CV-A16 and CV-A6 during the 3 years. Stool had a higher diagnostic yield than rectal and throat swabs . Detection rates reached 93% when testing stools followed by throat swabs if stools were negative, and 89% when testing rectal swabs followed by throat swabs if rectal swabs were negative. Conclusions Our results provide a virological benchmark for future surveillance and diagnostics. Continuous comprehensive virological surveillance is essential, especially after implementation of the EV-A71 vaccine in China, to monitor serotype replacement and the vaccine's impact.收起

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