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作者

白波 刘玉红 刘海青

作者单位

Department of Neurobiology, Taishan Medical College, Taian 271016, China

刊名

NEUROSCIENCE BULLETIN

年份

2007

卷号

Vol.23 No.3

页码

180-184

ISSN

1673-7067

关键词

nitric oxide apelin receptor pain caudate nucleus reverse transcription polymerase chain reaction

摘要

Objective To investigate the effect of nitric oxide on the expression of apelin receptor mRNA, as well as their correlation, in the caudate nucleus of rat. Methods L-Arginine , NG-nitro-L-arginine methyl ester and normal saline was separately microinjected into rat caudate nucleus. Expressions of neuronal NO synthase mRNA and apelin receptor mRNA were detected by RT-PCR at 4, 8, 12, 24 and 48 h after microinjection, and their correlation was determined. Results The expressions of nNOS mRNA an...更多

Objective To investigate the effect of nitric oxide on the expression of apelin receptor mRNA, as well as their correlation, in the caudate nucleus of rat. Methods L-Arginine , NG-nitro-L-arginine methyl ester and normal saline was separately microinjected into rat caudate nucleus. Expressions of neuronal NO synthase mRNA and apelin receptor mRNA were detected by RT-PCR at 4, 8, 12, 24 and 48 h after microinjection, and their correlation was determined. Results The expressions of nNOS mRNA and apelin receptor mRNA were both significantly increased after microinjection of L-Arg, but significantly decreased after microinjection of L-NAME compared with the NS control group. The nNOS mRNA had a positive correlation with the expression of apelin receptor mRNA after microinjection of L-Arg and L-NAME. Conclusion The activity of NOS in the central nervous system, especially in the caudate nucleus, is one of the key factors for NO to exert many kinds of biological actions, such as modulation of central pain, as a neurotransmitter. The neurobiological action of NO in rat caudate nucleus may be associated with apelin receptors.收起

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