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作者单位

College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian, 271018, China College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, China Department of Basic Medical Sciences, Taishan Medical College, Taian, 271000, China Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, 271018, China

刊名

Virus Research

年份

2019

卷号

Vol.270

ISSN

0168-1702

关键词

3CD 3Dpol DHAV-1 NLS

摘要

• The DHAV-1 3Dpol entered into nucleus during viral propagation. • The basic residue 17PRKTAYMRS25 in DHAV-1 3Dpol was proved as a functional NLS. • 18R, 19K and 24R are essential for maintaining the nuclear targeting activity of 3Dpol. • Exchange between 24R and 24K had no impact on cellular localization of 3Dpol. The nuclear localization signals were usually composed of basic residues and played an important role in delivery of genomes and structural protein into nucleus. In this research, ...更多

• The DHAV-1 3Dpol entered into nucleus during viral propagation. • The basic residue 17PRKTAYMRS25 in DHAV-1 3Dpol was proved as a functional NLS. • 18R, 19K and 24R are essential for maintaining the nuclear targeting activity of 3Dpol. • Exchange between 24R and 24K had no impact on cellular localization of 3Dpol. The nuclear localization signals were usually composed of basic residues and played an important role in delivery of genomes and structural protein into nucleus. In this research, we identified that 3Dpol/3CD entered into nucleus during viral propagation of duck hepatitis A virus type 1 . To investigate the reason that 3Dpol/3CD entered into nucleus, the amino acid sequence of 3CD was analyzed through NLS Mapper program. The basic region 17PRKTAYMRS25 was subsequently proved to be a functional NLS to guide 3Dpol/3CD into nucleus. 18R, 19K and 24R were found essential for maintaining the nuclear targeting activity, and exchange between 24R and 24K had no impact on cellular localization of 3Dpol. Since the entry of 3Dpol/3CD into nucleus was essential for shutoff of host cell transcription and maintaining the viral propagation of picornavirus numbers, our study provided new insights into the mechanism of DHAV-1 propagation.收起

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