Key Laboratory of Atherosclerosis in Universities of Shandong Province, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR China. Electronic address: scqin@sdfmu.edu.cn.Key Laboratory of Atherosclerosis in Universities of Shandong Province, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR China.Taishan Institute for Hydrogen Biomedicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR ChinaAffiliations
Aims: Oxidized phospholipids are formed as a result of oxidative stress, which potentially mediate multiple pathological effects. We aimed to evaluate the effects of hydrogen on OxPLs in vivo and the underlying mechanism. Main methods: Rats were randomly assigned to three groups: control group fed with a chow diet, model group fed with a high-fat diet, and H 2 -treated group fed with a high-fat diet and treated by 4% H 2 inhalation for ten weeks. OxPLs in liver and plasma were analyzed by Liq...更多
Aims: Oxidized phospholipids are formed as a result of oxidative stress, which potentially mediate multiple pathological effects. We aimed to evaluate the effects of hydrogen on OxPLs in vivo and the underlying mechanism. Main methods: Rats were randomly assigned to three groups: control group fed with a chow diet, model group fed with a high-fat diet, and H 2 -treated group fed with a high-fat diet and treated by 4% H 2 inhalation for ten weeks. OxPLs in liver and plasma were analyzed by Liquid chromatography-mass spectrometry. High-density lipoprotein was separated by ultracentrifugation. A proteomic analysis was performed to reveal the alternation of HDL protein composition and he antioxidant capacity of HDL was tested by low-density lipoprotein oxidation experiment. Furthermore, the activity or expression of HDL-associated enzymes were evaluated. Key findings: Inhalation of 4% H 2 decreased the accumulation of OxPLs in rats. In vitro tests revealed that the different concentrations of H 2 did not inhibit the formation of OxPLs mediated by non-enzymatic oxidation. H 2 inhalation altered the components and enhanced the anti-oxidative capacity of HDL in rats fed with a high-fat diet. Further experiments showed that H 2 significantly regulated the activity of lipoprotein-associated phospholipase A 2 , paraoxonase-1, and the expression of lecithin:cholesterol acyltransferase. Significance: Our findings revealed that H 2 may reduce the OxPLs levels through its influence on HDL-associated enzymes that can act on OxPLs, suggesting that H 2 can be used in alleviating diseases related to lipid peroxidation due to oxidative stress.收起
