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作者

Ren-feng Zhang Yu-xia Man Yuan-yuan Bai Chun-hong Shao Chun-mei Liu Cong-hui Wang Yong-xing Lei Yong Wang Yan Jin

作者单位

Corresponding author. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China LiaochengDongchangfu People's Hospital, Liaocheng, China Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou, China School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China Corresponding author. Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China Corresponding author. Shanghai ZJ Bio-Tech Co.,Ltd., China

刊名

Journal of Microbiology, Immunology and Infection

年份

2021

卷号

Vol.54 No.6

页码

1179-1183

ISSN

1684-1182

关键词

Clostridioides difficile Multilocus sequence typing PCR RT027 Whole genome sequence Antimicrobial resistance China

摘要

Background: The rapid spread of C. difficile 027 has become one of the leading threats of healthcare-associated infections wordwild. However, C. difficile 027 infections have rarely been reported in China. The objective of this study was to strengthen the understanding of the molecular characterizations of C. difficile 027 in China. Methods: In this study, stool specimens from 176 suspected CDI cases were collected from 1 Jan 2018 to 30 Jun 2019. These specimens were measured by GeneXpert test a...更多

Background: The rapid spread of C. difficile 027 has become one of the leading threats of healthcare-associated infections wordwild. However, C. difficile 027 infections have rarely been reported in China. The objective of this study was to strengthen the understanding of the molecular characterizations of C. difficile 027 in China. Methods: In this study, stool specimens from 176 suspected CDI cases were collected from 1 Jan 2018 to 30 Jun 2019. These specimens were measured by GeneXpert test and C.difficile colonies were identified and analyzed. Results: There were five samples positive for tcdA, tcdB, binary toxin genes and had deletions in tcdC gene. These five Clostridioides difficile isolates belonged to ST1 and confirmed as Clostridioides difficile 027 strains by PCR ribotyping. Through using whole genome sequencing, , we found that these five strains were closely clustered into the same predominant evolutionary branch and were highly similar to C. difficile 027 strain R20291. Antimicrobial susceptibility testing result showed they were highly resistant to fluoroquinolones. Conclusions: In Our study, five C. difficile 027 isolates were identified and characterized using MLST, PCR ribotyping and whole genome sequencing. We proposed that C. difficile 027 infections are probably neglected in China. Further epidemiological studies across the country together with the introduction of routine diagnostic testing and multi-center or national level surveillance are needed to ascertain the size of this potentially significant problem.收起

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