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作者

Li, Xianduo Tang, Guanbao Guo, Xuewen Men, Tongyi

作者单位

Department of Urology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, 250014, China School of Medicine, Shandong University, Jinan, 250012, China Department of Urology, First Affiliated Hospital of Shandong First Medical University, Jinan, 250014, China

刊名

Acta Medica Mediterranea

年份

2021

卷号

Vol.37 No.6

页码

2995

ISSN

0393-6384

分类号

616.9, 610

摘要

Objective: To investigate the correlation between the expression of thyroid hormone receptor interactor 13 and the clinicopathological characteristics of bladder cancer, along with the influence of TRIP13 on the biological behavior of bladder cancer cells. Methods: From January 2013 to February 2015, 38 samples of bladder cancer tissue were selected for inclusion in this study from those that were frozen in our hospital, along with 18 samples of normal bladder mucosa from patients in our hospit...更多

Objective: To investigate the correlation between the expression of thyroid hormone receptor interactor 13 and the clinicopathological characteristics of bladder cancer, along with the influence of TRIP13 on the biological behavior of bladder cancer cells. Methods: From January 2013 to February 2015, 38 samples of bladder cancer tissue were selected for inclusion in this study from those that were frozen in our hospital, along with 18 samples of normal bladder mucosa from patients in our hospital who had undergone surgery for benign diseases during the same time period. An immunohistochemical method was employed to detect the expression of TRIP13 in the bladder cancer tissues and the normal bladder mucosa tissues, and to analyze the correlation between its expression level and the clinicopathological characteristics and prognosis of the bladder cancer patients. At the same time, the human bladder cancer cell line T24 was collected for routine cultivation; its logarithmic growth phase was randomly divided into a blank control group, a negative control group, and a siRNA group. Small interfering RNA against TRIP13 were added to the cells in the siRNA group; they were then transfected with liposomes and introduced into T24 cells. The negative control group was transfected with irrelevant siRNA. All three groups of T24 cells were implanted in 6-well plates, at a density of 5×105/well, one day before transfection. The next day, the cells were transfected when the degree of cell fusion reached 70%; the serum and antibiotics were replaced 5 hours later. The three groups of T24 cellsclone formation rates, migration distances, and cell proliferation inhibition rates were compared at 24 h, 48 h, 72 h, and 96 h. Results: The positive expression rate of TRIP13 in the bladder cancer tissues was found to be 60.53% , significantly higher than that of TRIP13 in the normal bladder mucosa tissues, at 27.78% . The 5-year survival rate of TRIP13-positive-expression patients was found to be 30.43% , which was significantly lower than that of the TRIP13-negative-expression patients, at 60% . The cloning rate of T24 cells in the siRNA group was significantly lower than that in the blank control group, while there was no statistically significant difference between the blank control group and the negative control group in this regard . The migration distance of T24 cells in the siRNA group was significantly lower than that in the blank control group, while the blank control group was not different from the negative control group to a statistically significant degree . Conclusion: The expression level of TRIP13 in bladder cancer tissue is elevated, and its expression level is closely related to the depth of infiltration, lymph node metastasis, and TNM stage of the patient. TRIP13 is expected to become an important reference index for use to evaluate the prognosis of bladder cancer patients. In addition, TRIP13 has been found to play an important role in the behavior of bladder cancer cells; reducing its expression, therefore, holds the potential to significantly inhibit the proliferation, migration, and clonal formation of bladder cancer cells.收起

学科

【SCI学科】MEDICINE, GENERAL & INTERNAL 【ESI学科】CLINICAL MEDICINE

影响因子

2018年:0.835;2017年:0.751;2016年:0.196;2015年:0.154;2014年:0.113

文献类型

期刊