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作者

Zhen Xiong Zhao Sai Zhen Chen Zhe Lin Xia Yu Bin Xu Ling Ling Zhang Shan Ming Tian Qing Fan

作者单位

Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, PR China Taizhou Central Hospital (Taizhou University Hospital), Taizhou, Zhejiang, 317700, PR China

刊名

Journal of Pharmaceutical and Biomedical Analysis

年份

2022

页码

114576

ISSN

0731-7085

关键词

Nitrosamines Colorectal cancer GC-MS High nitrite diet Gut microbiota NDMA

摘要

N-nitrosamines are common toxic substances that have a strong correlation with many human diseases, such as liver damage and cancer. However, there is a lack of studies on methods involving the detection of NAs in biological samples, possibly owing to the interference of complex biological matrices and the influence of endogenous NAs. In this work, solid-phase extraction with mixed solid phases and adsorption sedimentation were used to successfully establish a gas chromatography–mass spectromet...更多

N-nitrosamines are common toxic substances that have a strong correlation with many human diseases, such as liver damage and cancer. However, there is a lack of studies on methods involving the detection of NAs in biological samples, possibly owing to the interference of complex biological matrices and the influence of endogenous NAs. In this work, solid-phase extraction with mixed solid phases and adsorption sedimentation were used to successfully establish a gas chromatography–mass spectrometry method for detecting eight NAs in rat faeces. Chromatographic separation of analytes was performed with Agilent VF-WAXms GC columns. The LLOQs of eight NAs were set to the concentration of 0.5 ng/g and the obtained standard curves were linear, and correlation coefficients were ≥ 0.99 for samples with concentration ranges of 0.5–500 ng/g. The inter and intra-assay precisions were < 15% for all analytes in the quality control samples, and the accuracies ranged from 88.67% to 108.33%. The extraction recoveries were above 78.56% for seven NAs, and a significant matrix effect was not observed. The application of this method revealed that the levels of NAS in the faeces of rats with colorectal cancer were higher than those of normal rats. Additionally, the effect of a high nitrite diet on NAs in faeces was analysed; the results confirmed that a high nitrite diet might contribute to an abnormal increase in NAs. Our work provides an analytical method for further in vivo study of NAs. Furthermore, a pilot study on the relationship between NAs and colorectal cancer was completed.收起

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