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作者

Li, Xueli Cheng, Weidong Li, Juan Guo, Xianling Guo, Cunju Meng, Xianhui Sun, Shenggang Wang, Lexin

作者单位

School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia Union Hospital, Tongji Medical College, HuaZhong University of Science and Technology, Wuhan, 430022, China Department of Neurology, Liaocheng People's Hospital, Liaocheng, Shandong Province, 252000, China Department of Neurology, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical College, Liaocheng Shandong, 252000, China

刊名

Clinical and Investigative Medicine

年份

2008

卷号

Vol.31 No.5

页码

E258

ISSN

0147-958X

摘要

Objectives: The protective effect of estrogen on the neurons in Parkinson's disease is unclear. The present study aimed to investigate the effect of estrogen on the apoptosis and dopaminergic function on a cultured cell model of PD. Methods: The PD model was established by addition of 1-methyl-4- phenylpyridinium to PC12 cell culture. Estrogen was added to cell groups with MPP+ , and without MPP+ . Cell viability, content of tyrosine hydroxylase , apoptosis ratio, expression of apoptosis-suppr...更多

Objectives: The protective effect of estrogen on the neurons in Parkinson's disease is unclear. The present study aimed to investigate the effect of estrogen on the apoptosis and dopaminergic function on a cultured cell model of PD. Methods: The PD model was established by addition of 1-methyl-4- phenylpyridinium to PC12 cell culture. Estrogen was added to cell groups with MPP+ , and without MPP+ . Cell viability, content of tyrosine hydroxylase , apoptosis ratio, expression of apoptosis-suppression protein Bcl-x and apoptosis-acceleration protein IL-1 beta converting enzyme were measured. Results: Cell viability in the Estrogen+MPP+ group was similar to the control group but was higher than in the MPP+ group . The apoptosis ratios in the Estrogen+ MPP+ group , and the control group , were also similar, but it was lower than in the MPP+ group . Concentrations of Bcl-x were higher in the Estrogen+MPP+ group, whereas ICE concentrations were lower than in the MPP+ group . Conclusions: Estrogen suppresses apoptosis and improves cell viability in MPP+ induced injuries in the PC12 cells. The beneficial effects of estrogen on the PD model are due to the suppression of pro-apoptotic protein ICE, and stimulation of anti-apoptotic protein Bcl-x.收起

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