Rapid detection of the emerging tick-borne Tamdy virus by TaqMan-based real-time reverse transcription PCR

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作者单位: International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, N20 W10 Kita-ku, Sapporo 001-0020, Japan Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China National Virus Reference Laboratory, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland College of Life Sciences & Technology, Inner Mongolia Normal University, Hohhot, Inner Mongolia 010022, China School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China

关键词: Tick-borne virus Tamdy virus Bunyaviruses Real-time reverse transcription PCR

摘要: Tamdy virus is an emerging zoonotic tick-borne arbovirus in the genus Orthonairovirus. Reports of human infections with TAMV have been increasing and development of a rapid detection assay is thus urgently required. In the present study, singleplex and dual-target real-time reverse transcription PCR assays were established for the detection of TAMV. Sensitivity and specificity were evaluated, which demonstrated high sensitivity for both the singleplex and dual-target qRT-PCR assays with no cro...更多; Tamdy virus is an emerging zoonotic tick-borne arbovirus in the genus Orthonairovirus. Reports of human infections with TAMV have been increasing and development of a rapid detection assay is thus urgently required. In the present study, singleplex and dual-target real-time reverse transcription PCR assays were established for the detection of TAMV. Sensitivity and specificity were evaluated, which demonstrated high sensitivity for both the singleplex and dual-target qRT-PCR assays with no cross-reaction with common bunyaviruses and tick-borne viruses. The TaqMan-based qRT-PCR methodology established in this study can be employed for epidemiological surveillance and pathogenesis studies of TAMV.收起

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