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作者

Chaoguang Luan, Ying Xu

作者单位

Affiliations ¹ General Surgery, Ji 'nan Municipal Three Hospitals, Ji'nan, Shandong, China. ² Postdoctoral Centre, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Science, Ji'nan, Shandong, China.

刊名

Cell biology international

年份

2022

ISSN

1095-8355

关键词

cholangiocarcinoma hepatocellular carcinoma matrix metalloproteinase single-nucleotide polymorphism telocytes.

摘要

Telocytes have crucial functions to promote the metastasis of hepatocellular carcinoma by over-expressing matrix metalloproteinases , but the mechanism by which TCs secrete MMPs in the genome is still unknown. We first cultured and isolated primary TCs from distinct liver cancer tissues and hepatic hemangioma surrounding tissues . Their whole exon genes were tested by Illumina HiSeq family of platforms and by high-throughput sequencing as well as variant mutations. Moreover, immunohistochemist...更多

Telocytes have crucial functions to promote the metastasis of hepatocellular carcinoma by over-expressing matrix metalloproteinases , but the mechanism by which TCs secrete MMPs in the genome is still unknown. We first cultured and isolated primary TCs from distinct liver cancer tissues and hepatic hemangioma surrounding tissues . Their whole exon genes were tested by Illumina HiSeq family of platforms and by high-throughput sequencing as well as variant mutations. Moreover, immunohistochemistry, Western blot, and quantitative reverse transcription-polymerase chain reaction assays were utilized to assess the expression of MMPs. The perniciousness of signal-nucleotide polymorphism mutations of proteins were predicted by the Polyphen-2 database. Divergent expression and overall survival of MMPs was screened by StarBase-Pan Cancer plate; and MMPs associated signaling pathways were found by Kyoto Encyclopedia Genes and Genomes. The 'competing endogenous RNA ' network was constructed by Cytoscape software. We found that 12 specific types of SNP mutations related to 5 types of MMPs occurred in TCs of liver malignant tumors as a potential result of MMP1, MMP9, and MMP17 overexpression. High levels of MMP1, MMP7, and MMP9 represented poor OS in HCC, and an interactive network of MMPs is shown. Allele shifts of C/T and G/C in MMP9 were risk factors for TCs in HCC by the prediction of the Polyphen-2 Database. ) mutation might be a genetic mechanism of upregulating MMP9 in TCs.收起

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