Objective To explore the effects of intraperitoneal injection of interleukin-37on myocardial in⁃ jury in sepsis mice. Methods Thirty C57BL/6J mice were randomly divided into three groups：control group，LPS group，and IL-37 group，with 10 in each group. The mice in the LPS group were intraperitoneally injected with 20 mg/kg lipopolysaccharide，the mice in the IL-37 group were intraperitoneally injected with 20 mg/kg LPS and 1 mg/kg IL- 37，and the mice in the control group were intraperitoneally injec...更多
Objective To explore the effects of intraperitoneal injection of interleukin-37on myocardial in⁃ jury in sepsis mice. Methods Thirty C57BL/6J mice were randomly divided into three groups：control group，LPS group，and IL-37 group，with 10 in each group. The mice in the LPS group were intraperitoneally injected with 20 mg/kg lipopolysaccharide，the mice in the IL-37 group were intraperitoneally injected with 20 mg/kg LPS and 1 mg/kg IL- 37，and the mice in the control group were intraperitoneally injected with an equal volume of sterile normal saline. The car⁃ diac function of the mice in each group was evaluated by cardiac ultrasound，and the degree of pathological damage of the myocardial tissues of the mice in each group was evaluated by HE staining. The expression levels of inflammatory factors IL-1β，IL-6 and tumor necrosis factor-αin the myocardial tissues were detected by immunohistochemistry. The protein expression levels of NOD-like receptor protein 3inflammasome and its related signaling pathways were an⁃ alyzed by immunohistochemistry and Western blotting. Results Compared with the control group，both left ventricle ejection fractionand fractional shorteningin the LPS group decreased. Compared with LPS group，both LVEF and FS in the IL-37 group increased. Compared with the control group，the myocardial tissue structure in LPS group was disordered，with the disruptive sarcolemma，congestive and oedematous myocardial inter⁃ stitium. Compared with the LPS group，the heart function and myocardial histopathological injury were significantly im⁃ proved in the IL-37 group. The expression levels of IL-1β were 20. 12 ± 1. 89，1. 00 ± 0. 56，8. 09 ± 2. 89 in the LPS group，control group and IL-37 group，the expression levels of IL-6 were 36. 56 ± 5. 17，1. 00 ± 0. 25，9. 92 ± 1. 37，re⁃ spectively，and the expression levels of TNF-α were 20. 87 ± 6. 35，1. 00 ± 0. 44，and 6. 23 ± 1. 29，respectively. The ex⁃ pression levels of IL-1β，IL-6 and TNF-α in the myocardium in the LPS group were higher than those in the control group ，and those were lower in the IL-37 group than in the LPS group. NLRP3 protein expression levels were 6. 42 ± 0. 16 in the LPS group，1. 00 ± 0. 21 in the control group，and 5. 39 ± 0. 11 in the IL-37 group，respec⁃ tively. NLRP3 protein expression was higher in the LPS group than in the control group，and NLRP3 protein ex⁃ pression was lower in the IL-37 group than in the LPS group. The relative expression levels of NLRP3 protein in the LPS group，control group and IL-37 group were 3. 77 ± 0. 22，1. 00 ± 0. 04，and 1. 97 ± 0. 13，respectively. The rela⁃ tive expression levels of Caspase-1 protein were 3. 04 ± 0. 18，1. 00 ± 0. 07，and 1. 62 ± 0. 20，respectively. The relative expression levels of ASC protein were 2. 17 ± 0. 12，1. 00 ± 0. 03 and 0. 77 ± 0. 13，respectively. The expression levels of NLRP3，Caspase-1，and ASC protein in the myocardial tissues in the LPS group were higher than those in the control group，while those were lower in the IL-37 group than in the LPS group. Conclusions IL- 37 may ameliorate the myocardial injury in sepsis mice by inhibiting the NLRP3 inflammasome收起
