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作者

Wang, Lin Wang, Ning Zhao, Yinghui Lu, Gang

作者单位

^eDepartment of Pathogen Biology, School of Basic Medical Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, 6699 Qingdao Road, Shandong, Jinan, 250062, China ^aDepartment of Epilepsy Center, Ji Nan Children's Hospital, Shandong, Jinan, 250022, China ^dDepartment of Pathogen Biology, School of Basic Medical Sciences, The Second Affiliated Hospital of Shandong First Medical University & Shandong Academy of Medical Sciences, Shandong, Taian, 271000, China ^cDepartment of Pathogen Biology, School of Basic Medical Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Shandong, Jinan, China ^bDepartment of Clinical Laboratory, Qingdao Third People's Hospital, Shandong, Qingdao, 266041, China

刊名

Oncology Letters

年份

2022

卷号

Vol.23 No.5

页码

149

ISSN

1792-1074

关键词

Cancer‑associated miRNAs MiRNA‑seq Suppression Toxoplasma gondii Tumor suppressor genes

摘要

Throughout the world, numerous individuals are infected with Toxoplasma gondii, which may improve immunity against cancer. Furthermore, microRNAs may be differentially expressed in the host upon infec‑ tion with T. gondii. In the present study, RNA‑sequencing analysis and reverse transcription‑quantitative PCR revealed that miR‑429‑3p, miR‑145a‑5p, miR‑211‑5p, miR‑31‑3p and miR‑135a‑5p were determined to be downregulated, while miR‑21a‑3p, miR‑135b‑5p, miR‑210‑5p and miR‑146‑3p were upregulated...更多

Throughout the world, numerous individuals are infected with Toxoplasma gondii, which may improve immunity against cancer. Furthermore, microRNAs may be differentially expressed in the host upon infec‑ tion with T. gondii. In the present study, RNA‑sequencing analysis and reverse transcription‑quantitative PCR revealed that miR‑429‑3p, miR‑145a‑5p, miR‑211‑5p, miR‑31‑3p and miR‑135a‑5p were determined to be downregulated, while miR‑21a‑3p, miR‑135b‑5p, miR‑210‑5p and miR‑146‑3p were upregulated in mice post‑infection with T. gondii. Antitumor genes [TNF receptor superfamily member 11b, large tumor suppressor kinase 2 and Lats1] were identified as targets of miR‑429‑3p, miR‑145a‑5p, miR‑211‑5p, miR‑31‑3p and miR‑135a‑5p with a luciferase reporter assay. In addition, the protein levels of Lats2 and Lats1 were detected to be higher in T. gondii‑infected mice than in the control group. Therefore, these results provide favorable evidence for the suppression of cancer upon T. gondii infection and may give novel ideas for the treatment of tumors.收起

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