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作者

Jingyi Sun Yanan Song Mengyue Wang Peng Zhao Feng Gao Junqi Li Mingfeng Yang Hui Yuan Baoliang Sun Ying Wang

作者单位

Affiliations ¹ Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China. ² The Second Affiliated Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China. ³ Qingdao Medical College of Qingdao University, Qingdao, Shandong 266021, China. ⁴ Department of Neurology, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, Hainan 570102, China.

刊名

ACS applied materials & interfaces

年份

2022

卷号

Vol.14 No.32

页码

37088-37100

ISSN

1944-8252

关键词

SERS acupuncture cerebrospinal fluid living cell miRNA subarachnoid hemorrhage.

摘要

Quantitative analysis of microRNAs in a noninvasive manner is of vital importance for disease diagnosis and prognosis evaluation. However, conventional strategies for realizing accurate, simple, and sensitive detection of target molecules are still a challenge, especially for miRNAs due to their low abundance and susceptibility in the complex biological environment. Here, a novel surface-enhanced Raman scattering strategy was established for quantitative detection and monitoring of miRNA-21-5p...更多

Quantitative analysis of microRNAs in a noninvasive manner is of vital importance for disease diagnosis and prognosis evaluation. However, conventional strategies for realizing accurate, simple, and sensitive detection of target molecules are still a challenge, especially for miRNAs due to their low abundance and susceptibility in the complex biological environment. Here, a novel surface-enhanced Raman scattering strategy was established for quantitative detection and monitoring of miRNA-21-5p in living cells and in vivo cerebrospinal fluid by applying hairpin DNA -conjugated gold nanostars SERS probes combined with acupuncture-based technology. This strategy enabled ultrasensitive exploration toward miR-21-5p in a wide range from 1 fM to 100 pM in cell lysates. Moreover, SERS analysis facilitated the detection and long-term monitoring for in vivo miR-21-5p noninvasively. This developed strategy promises to offer a powerful method for the analysis of multiple biomolecules in single cells and living bodies.收起

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