获取途径

作者

Houmin Wan Lianzhen Wang Bin Huo Zhongpeng Qiao Yingli Zhang

作者单位

Municipal Hospital Affiliated to Shandong First Medical University, Jinan, 250031, Shandong Province, China ¹Department of Gastrointestinal Surgery, The NO ²Department of Obstetrics, The NO

刊名

Heliyon

年份

2023

页码

e21061

ISSN

2405-8440

关键词

CIZ1 miR-339-3p FBXL19-AS1 Gastric cancer

摘要

Gastric cancer remains a prevalent malignancy with high morbidity and mortality. CDKN1A interacting zinc finger protein 1 has been demonstrated to have oncogenic functions in the development of cancers. We detected CIZ1 expression via quantitative real-time PCR . The protein level of CIZ1 was measured through Western blot. We noticed that CIZ1 expression was markedly enhanced in GC cells. Furthermore, functional experiments including colony formation assay, EdU staining assay, transwell assay,...更多

Gastric cancer remains a prevalent malignancy with high morbidity and mortality. CDKN1A interacting zinc finger protein 1 has been demonstrated to have oncogenic functions in the development of cancers. We detected CIZ1 expression via quantitative real-time PCR . The protein level of CIZ1 was measured through Western blot. We noticed that CIZ1 expression was markedly enhanced in GC cells. Furthermore, functional experiments including colony formation assay, EdU staining assay, transwell assay, TUNEL staining assay and flow cytometry analysis uncovered that CIZ1 silencing attenuated cell malignant phenotypes in GC. Through bioinformatics tools and mechanism assays, we explored the up-stream mechanism of CIZ1 and determined that CIZ1 was modulated by FBXL19 antisense RNA 1 and microRNA-339-3p . Additionally, miR-339-3p exerted a negative role on GC development in vitro, and FBXL19-AS1 depletion also had the inhibitory impacts on the progression of GC in vitro. Eventually, the finding that CIZ1 overexpression reversed the effects of FBXL19-AS1 silencing on GC development was validated by rescue assays. In a word, CIZ1 functioned as a tumor promoter in GC, indicating that CIZ1 might be a promising target for GC treatment.收起

文献类型

期刊

浏览量

6