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作者

Yuan Xu Yuhui Li Jingyong Zhang Pingping Cai

作者单位

³Medical Equipment Department, Shandong Cancer Hospital, Jinan, Shandong 250117, PR China ²Traditional Chinese Medicine Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, PR China ¹First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, PR China ⁴Vascular Surgery Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, PR China

刊名

Tissue and Cell

年份

2025

卷号

Vol.93

页码

102763

ISSN

0040-8166

摘要

Background Our purpose is to explore the influence of Hua Yu Xiao Zheng decoction on the senescence of ectopic endometrial stromal cells in endometriosis as well as its relevant mechanisms. Methods The proliferation, apoptosis, migration, and invasion of primary ectopic ESCs was assessed utilizing EdU assay, flow cytometry, and transwell assays. Moreover, the effects of HYXZ decoction on oxidative stress were evaluated using DCFH-DA probe and via measuring MDA, GSH, SOD and GPx levels. The fun...更多

Background Our purpose is to explore the influence of Hua Yu Xiao Zheng decoction on the senescence of ectopic endometrial stromal cells in endometriosis as well as its relevant mechanisms. Methods The proliferation, apoptosis, migration, and invasion of primary ectopic ESCs was assessed utilizing EdU assay, flow cytometry, and transwell assays. Moreover, the effects of HYXZ decoction on oxidative stress were evaluated using DCFH-DA probe and via measuring MDA, GSH, SOD and GPx levels. The function of HYXZ decoction on cell senescence were explored through utilizing SA-β-gal staining and measuring the cumulative population doubling level and the average telomere length. The protein expression was measured using western blotting analysis. Endometriosis in rats was surgically induced, and the efficacy and mechanism of HYXZ decoction were determined in vivo. Results HYXZ decoction inhibited the growth, migration, and invasion of ectopic ESCs, but induced oxidative stress and senescence. HYXZ decoction inhibited PI3K/AKT signaling in ectopic ESCs. PI3K-AKT signaling pathway activator significantly reversed the inhibitory effects of HYXZ decoction on ectopic ESCs. In rats with endometriosis, oral administration of HYXZ decoction inhibited lesion volume alone with the increased oxidative stress and cell senescence, as well as the decreased PI3K/AKT activity. Conclusion HYXZ decoction might repress the growth and migration of ectopic ESCs, and induce oxidative stress and senescence through suppressing PI3K/AKT signaling. This finding indicates that HYXZ decoction may be a potential therapeutic drug against endometriosis.收起

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