Objective: To compare the diagnostic efficacy of acid-fast smear of Mycobacterium tuberculosis, Gene Xpert MTB/RIF and metagenomic next-generation sequencing in pulmonary tuberculosis. Methods: The clinical data of 175 patients with suspected pulmonary tuberculosis attending the Public Health Clinical Center of Shandong Province from March 2021 to April 2023 were retrospec-tively analyzed, and all the included cases underwent sputum antacid smear, alveolar lavage fluid antacid smear, sputum cul...更多
Objective: To compare the diagnostic efficacy of acid-fast smear of Mycobacterium tuberculosis, Gene Xpert MTB/RIF and metagenomic next-generation sequencing in pulmonary tuberculosis. Methods: The clinical data of 175 patients with suspected pulmonary tuberculosis attending the Public Health Clinical Center of Shandong Province from March 2021 to April 2023 were retrospec-tively analyzed, and all the included cases underwent sputum antacid smear, alveolar lavage fluid antacid smear, sputum culture, alveolar lavage fluid culture, alveolar lavage fluid Gene Xpert MTB/RIF, and mNGS. Positive rates of antacid smear, Mycobacterium tuberculosis culture, Gene Xpert MTB/RIF and mNGS for the diagnosis of tuberculosis were compared. Results: A total of 126 cases of TB were diagnosed in 175 patients with suspected TB. The sensitivity of Gene Xpert MTB/RIF in lavage fluid was 77.8%, and the positivity rate of mNGS in lavage fluid was 85.7%, which was higher than that of sputum culture method and lavage culture method , and the positivity rate of Gene Xpert MTB/RIF and mNGS was higher than that of culture method in smear-negative patients with TB. For smear-negative TB patients, Gene Xpert MTB/RIF and mNGS were more positive than culture method Conclusion: mNGS and Gene Xpert MTB/RIF are important in the diagnosis of TB, and greatly increase the detection rate of smear-negative TB.收起
