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作者

陈淑芳 姜淑娟 李 晶 王骏仁

作者单位

山东大学齐鲁医学院，山东 济南 山东省公共卫生临床中心，呼吸与危重症医学科，山东 济南 山东第一医科大学附属省立医院，呼吸与危重症医学科，山东 济南

刊名

Advances in Clinical Medicine

年份

2023

卷号

Vol.13 No.12

页码

19093-19100

ISSN

2161-8712

关键词

结核分枝杆菌 Gene Xpert MTB/RIF 宏基因组二代测序 诊断 Mycobacterium tuberculosis Gene Xpert MTB/RIF Metagenomic Next-Generation Sequencing Diagnosis

摘要

Objective: To compare the diagnostic efficacy of acid-fast smear of Mycobacterium tuberculosis, Gene Xpert MTB/RIF and metagenomic next-generation sequencing in pulmonary tuberculosis. Methods: The clinical data of 175 patients with suspected pulmonary tuberculosis attending the Public Health Clinical Center of Shandong Province from March 2021 to April 2023 were retrospec-tively analyzed, and all the included cases underwent sputum antacid smear, alveolar lavage fluid antacid smear, sputum cul...更多

Objective: To compare the diagnostic efficacy of acid-fast smear of Mycobacterium tuberculosis, Gene Xpert MTB/RIF and metagenomic next-generation sequencing in pulmonary tuberculosis. Methods: The clinical data of 175 patients with suspected pulmonary tuberculosis attending the Public Health Clinical Center of Shandong Province from March 2021 to April 2023 were retrospec-tively analyzed, and all the included cases underwent sputum antacid smear, alveolar lavage fluid antacid smear, sputum culture, alveolar lavage fluid culture, alveolar lavage fluid Gene Xpert MTB/RIF, and mNGS. Positive rates of antacid smear, Mycobacterium tuberculosis culture, Gene Xpert MTB/RIF and mNGS for the diagnosis of tuberculosis were compared. Results: A total of 126 cases of TB were diagnosed in 175 patients with suspected TB. The sensitivity of Gene Xpert MTB/RIF in lavage fluid was 77.8%, and the positivity rate of mNGS in lavage fluid was 85.7%, which was higher than that of sputum culture method and lavage culture method , and the positivity rate of Gene Xpert MTB/RIF and mNGS was higher than that of culture method in smear-negative patients with TB. For smear-negative TB patients, Gene Xpert MTB/RIF and mNGS were more positive than culture method Conclusion: mNGS and Gene Xpert MTB/RIF are important in the diagnosis of TB, and greatly increase the detection rate of smear-negative TB.收起

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