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作者

Shiwei Yang, Wenqi Hu, Xuezhi Xin, Guang Zhang

作者单位

Affiliations ¹ First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China. ² Department of Health Management, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Engineering Research Center of Health Management, Jinan, Shandong, China. ³ Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.

刊名

Chemical biology & drug design

年份

2025

卷号

Vol.106 No.4

页码

e70184

ISSN

1747-0285

关键词

HIF‐1α Skullcapflavone II colorectal cancer glycolysis invasion migration proliferation.

摘要

Our aim is to explore Skullcapflavone II function in colorectal cancer and delineate its relevant mechanisms. Malignant behaviors of HCT-116 and SW480 cells were assessed using colony formation, 5-ethynyl-2'-deoxyuridine staining, Annexin V/propidium iodide double staining, scratch and transwell assays. Changes in glycolysis were assessed by seahorse assay and western blot. The potential mechanism by which SFII inhibited the expression of hypoxia-inducible transcription factor-1alpha was explo...更多

Our aim is to explore Skullcapflavone II function in colorectal cancer and delineate its relevant mechanisms. Malignant behaviors of HCT-116 and SW480 cells were assessed using colony formation, 5-ethynyl-2'-deoxyuridine staining, Annexin V/propidium iodide double staining, scratch and transwell assays. Changes in glycolysis were assessed by seahorse assay and western blot. The potential mechanism by which SFII inhibited the expression of hypoxia-inducible transcription factor-1alpha was explored in vitro by molecular docking, surface plasmon resonance, quantitative reverse transcription polymerase chain reaction, western blot, immunofluorescence, cycloheximide chase assay, ubiquitination assay, and some rescue experiments. Besides, a xenograft model was performed to verify SFII function on colorectal cancer. We found that SFII inhibited the malignant behavior and glycolysis of colorectal cancer cells. Furthermore, SFII inhibited HIF-1α protein level in colorectal cancer cells via enhancing its ubiquitination. Moreover, HIF-1α overexpression attenuated SFII effect on the malignant behavior and glycolysis in colorectal cancer cells. In vivo, SFII inhibited tumorigenesis through repressing the HIF-1α-glycolysis pathway in BALB/c nude mice. SFII could suppress proliferation, migration, and invasion in colorectal cancer through inhibiting the HIF-1α-glycolysis pathway.收起

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