Affiliations 1 First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China. 2 Department of Health Management, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Engineering Research Center of Health Management, Jinan, Shandong, China. 3 Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.
Our aim is to explore Skullcapflavone II function in colorectal cancer and delineate its relevant mechanisms. Malignant behaviors of HCT-116 and SW480 cells were assessed using colony formation, 5-ethynyl-2'-deoxyuridine staining, Annexin V/propidium iodide double staining, scratch and transwell assays. Changes in glycolysis were assessed by seahorse assay and western blot. The potential mechanism by which SFII inhibited the expression of hypoxia-inducible transcription factor-1alpha was explo...更多
Our aim is to explore Skullcapflavone II function in colorectal cancer and delineate its relevant mechanisms. Malignant behaviors of HCT-116 and SW480 cells were assessed using colony formation, 5-ethynyl-2'-deoxyuridine staining, Annexin V/propidium iodide double staining, scratch and transwell assays. Changes in glycolysis were assessed by seahorse assay and western blot. The potential mechanism by which SFII inhibited the expression of hypoxia-inducible transcription factor-1alpha was explored in vitro by molecular docking, surface plasmon resonance, quantitative reverse transcription polymerase chain reaction, western blot, immunofluorescence, cycloheximide chase assay, ubiquitination assay, and some rescue experiments. Besides, a xenograft model was performed to verify SFII function on colorectal cancer. We found that SFII inhibited the malignant behavior and glycolysis of colorectal cancer cells. Furthermore, SFII inhibited HIF-1α protein level in colorectal cancer cells via enhancing its ubiquitination. Moreover, HIF-1α overexpression attenuated SFII effect on the malignant behavior and glycolysis in colorectal cancer cells. In vivo, SFII inhibited tumorigenesis through repressing the HIF-1α-glycolysis pathway in BALB/c nude mice. SFII could suppress proliferation, migration, and invasion in colorectal cancer through inhibiting the HIF-1α-glycolysis pathway.收起
