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作者

Yuan Li, Yuanyuan Sun, Yuxia Huang, Xiaoyi Hu, Ping Yuan

作者单位

Affiliations ¹ Department of Pulmonary and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China. ² Department of Cardio-pulmonary Circulation, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. ³ Department of Pulmonary and Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

刊名

Frontiers in genetics

年份

2025

卷号

Vol.16

页码

1674894

ISSN

1664-8021

关键词

circRNA idiopathic pulmonary artery hypertension miRNA molecular signature sex difference.

摘要

Background: Idiopathic pulmonary artery hypertension is a life-threatening condition with obvious sex differences. Recently, the interactions among circRNAs, miRNAs, and mRNAs were found to be significant parts in IPAH. Thus, we want to explore whether RNA networks exist in IPAH via sex differential analyses. Methods and materials: Illumina HiSeq was used to detect differentially expressed RNAs in peripheral blood mononuclear cells from six IPAH patients. Gene Ontology and Kyoto Encyclopedi...更多

Background: Idiopathic pulmonary artery hypertension is a life-threatening condition with obvious sex differences. Recently, the interactions among circRNAs, miRNAs, and mRNAs were found to be significant parts in IPAH. Thus, we want to explore whether RNA networks exist in IPAH via sex differential analyses. Methods and materials: Illumina HiSeq was used to detect differentially expressed RNAs in peripheral blood mononuclear cells from six IPAH patients. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were employed for the exhibition of differential expression levels of circRNAs, miRNAs, and mRNAs. The competing endogenous RNA networks among circRNAs, miRNAs, and mRNAs were constructed on the basis of the authoritative miRanda and TargetScan databases. Student's t-test was employed, and p < 0.05 was applied to show significance. Results: There were 220 DE circRNAs , 41 DE miRNAs , and 160 mRNAs in the female group compared with the male group, respectively. Through interaction analyses among circRNAs, miRNAs, and mRNAs, upregulated has-miR-1304-3p might be sponged to hsa-circ-0005723 and hsa-circ-0030276 and downregulated hsa-miR-6859-3p was probably sponged to circ DGKD, to modulate 21 downregulation genes and six upregulation genes, respectively. Conclusion: There were sex differences in a comprehensive circRNA-miRNA-mRNA regulatory network in IPAH.收起

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