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作者

Bin Wu Albert Gao Bin He Yun Chen Xiangfeng Kong Fayuan Wen Haijun Gao

作者单位

Department of Reproductive Medicine，Central Hospital Affiliated to Shandong First Medical University，Jinan，Shandong，P.R.China；Department of Physiology and Biophysics，College of Medicine，Howard University，Washington，District of Columbia，USA

刊名

动物模型与实验医学(英文版)

年份

2025

卷号

第8卷

期号

第4期

页码

649-661

关键词

AMPK ATP production gene expression mitochondria RNA-seq trophoblast

分类号

R321.4;R329.24;R342.2;R394.2;R977

摘要

Background:How AMP activated protein kinasesignaling regulates mito-chondrial functions and mitophagy in human trophoblast cells remains unclear.This study was designed to investigate potential players mediating the regulation of AMPK on mitochondrial functions and mitophagy by next generation RNA-seq.Methods:We compared ATP production in protein kinase AMP-activated catalytic subunit alpha 1/2knockdownand control BeWo cells using the Seahorse real-time ATP rate test,then analyzed gene expressio...更多

Background:How AMP activated protein kinasesignaling regulates mito-chondrial functions and mitophagy in human trophoblast cells remains unclear.This study was designed to investigate potential players mediating the regulation of AMPK on mitochondrial functions and mitophagy by next generation RNA-seq.Methods:We compared ATP production in protein kinase AMP-activated catalytic subunit alpha 1/2knockdownand control BeWo cells using the Seahorse real-time ATP rate test,then analyzed gene expression profiling by RNA-seq.Differentially expressed geneswere examined by Gene Ontologyanalysis and Kyoto Encyclopedia of Genes and Genomespathway enrichment.Then protein-protein interactionsamong mitochondria related genes were fur-ther analyzed using Metascape and Ingenuity Pathway Analysissoftware.Results:Both mitochondrial and glycolytic ATP production in AKD cells were lower than in the control BeWo cells,with a greater reduction of mitochondrial ATP production.A total of 1092 DEGs were identified,with 405 upregulated and 687downregulated.GO analysis identified 60 genes associated with the term‘mitochon-drion'in the cellular component domain.PPI analysis identified three clusters of mito-chondria related genes,including aldo-keto reductase family 1member B10 and B15,alanyl-tRNA synthetase 1,mitochondrial ribosomal protein S6,mitochondrial calcium uniporter dominant negative subunit betaand dihydrolipoamide branched chain transacylase E2。Conclusions:In summary,this study identified multiple mitochondria related genes regulated by AMPK in BeWo cells,and among them,three clusters of genes may po-tentially contribute to altered mitochondrial functions in response to reduced AMPK signaling.收起

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