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作者

Yutong Liu Hua Li Liang Zhang Jing Tong Huiyu Liu Xingyu Yan Lisheng Liu Cong Zhang

作者单位

⁷Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, China ³Department of Gynecology and Obstetrics, Ji'nan Maternity and Child Care Hospital, Jinan, Shandong 250001, China ¹Department of Reproductive Medicine, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200135, China ⁴Research Center of Translational Medicine, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250013, China ²Y. L., H. L., L. Z. J. T., these authors contributed equally to this work. ⁶State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China ⁵College of Life Sciences, Shandong Normal University, Jinan, Shandong, 250014, China

刊名

Molecular and Cellular Endocrinology

年份

2026

卷号

Vol.618

页码

112803

ISSN

0303-7207

摘要

Preeclampsia is a pregnancy-specific syndrome affecting over 8 million mother-infant pairs annually; while most prior studies on PE pathogenesis have focused on the placenta, decidualization is fundamental to placentation and subsequent pregnancy progression. Pyruvate dehydrogenase kinase-1 is a key regulator of metabolic switching, as it promotes glycolysis and inhibits oxidative phosphorylation. Although PDK1 is aberrantly upregulated in various tumors, its role in PE remains unclear. In thi...更多

Preeclampsia is a pregnancy-specific syndrome affecting over 8 million mother-infant pairs annually; while most prior studies on PE pathogenesis have focused on the placenta, decidualization is fundamental to placentation and subsequent pregnancy progression. Pyruvate dehydrogenase kinase-1 is a key regulator of metabolic switching, as it promotes glycolysis and inhibits oxidative phosphorylation. Although PDK1 is aberrantly upregulated in various tumors, its role in PE remains unclear. In this study, we first observed that PDK1 expression in decidual tissues was significantly lower in patients with severe PE than in normal pregnant women. In vitro, PDK1 expression increased significantly during the decidualization of human endometrial stromal cells, accompanied by upregulated mRNA levels of aerobic glycolysis-related genes and downregulated expression of the oxidative phosphorylation enzyme IDH2. Moreover, PDK1 knockdown impaired decidualization, significantly reduced LDHA and MCT4 expression, increased IDH2 levels, suppressed glucose uptake and lactate production, and inhibited trophoblast invasion. In vivo experiments in mice further confirmed that PDK1 expression increases with decidual progression during both normal pregnancy and pseudopregnancy, which is paralleled by enhanced glycolysis and reduced oxidative phosphorylation. Collectively, these findings indicate that PDK1 plays a critical role in decidualization through promoting glycolysis and inhibiting oxidative phosphorylation, with aberrant PDK1 expression potentially impairing decidualization and being associated with PE pathogenesis.收起

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