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作者

Miao-Miao Xu, Yun-Xin Yao, Hui Lyu, Yu Qian, Zhe-Zhe Tian, Yu-Chen Fan, Kai Wang

作者单位

Affiliations ¹ Department of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, China. ² Department of Serve Liver Disease, Shandong Public Health Clinical Center of Shandong University, Jinan, Shandong, China. ³ Department of Infectious Diseases, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong, China. ⁴ Hepatology Institute of Shandong University, Jinan, Shandong, China.

刊名

Journal of medical virology

年份

2026

卷号

Vol.98 No.9

页码

e71118

ISSN

1096-9071

关键词

DNA methylation HBV‐ACLF ICAM‐1 biomarkers short‐term prognosis.

摘要

Hepatitis B virus-related acute-on-chronic liver failure is associated with a high short-term mortality rate. Therefore, early and accurate prognostic prediction is crucial for precise clinical management. This study aims to investigate the expression patterns of intercellular adhesion molecule-1 and its predictive value for the short-term prognosis of patients with HBV-ACLF. The Methylight method was used to quantitatively detect ICAM-1 promoter methylation level in peripheral blood mononucle...更多

Hepatitis B virus-related acute-on-chronic liver failure is associated with a high short-term mortality rate. Therefore, early and accurate prognostic prediction is crucial for precise clinical management. This study aims to investigate the expression patterns of intercellular adhesion molecule-1 and its predictive value for the short-term prognosis of patients with HBV-ACLF. The Methylight method was used to quantitatively detect ICAM-1 promoter methylation level in peripheral blood mononuclear cells of 286 participants. Meanwhile, the mRNA and plasma expression levels of ICAM-1 were determined using RT-qPCR and ELISA, respectively. The ICAM-1 promoter methylation levels in PBMCs of HBV-ACLF patients were significantly lower than those in chronic hepatitis B patients and healthy controls , whereas the mRNA and plasma expression levels of ICAM-1 were markedly elevated. The ICAM-1 methylation levels in HBV-ACLF patients correlated with specific clinical parameters. Among HBV-ACLF patients, ICAM-1 methylation levels were significantly lower in the non-survivor groups at both 28 and 90 days. The study further revealed that ICAM-1 methylation level serves as an independent influencing factor for the prognosis of HBV-ACLF patients at 28 and 90 days. Based on ROC curve and Kaplan-Meier curves, ICAM-1 methylation levels demonstrated excellent performance in predicting 28- and 90-day mortality in patients with HBV-ACLF. In conclusion, patients with HBV-ACLF exhibit hypomethylation of the ICAM-1 promoter. The combination of ICAM-1 promoter methylation level and MELD score can effectively enhance the predictive ability for the short-term prognosis of HBV-ACLF patients.收起

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