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作者

Meng-Meng Li Teng Jiang Zhen Sun Qun Zhang Chen-Chen Tan Jin-Tai Yu Lan Tan

作者单位

Department of Neurology, Qingdao Municipal Hospital, Taishan Medical University, Qingdao, China Department of Neurology,Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, China Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Qingdao, China

刊名

Scientific Reports

年份

2014

页码

1-7

ISSN

2045-2322

关键词

MICRORNA TEMPORAL lobe epilepsy AMYGDALOID body CASPASES APOPTOSIS HIPPOCAMPUS (Brain) RATS as laboratory animals

摘要

The expression and functions of microRNAs (miRNAs) in chronic temporal lobe epilepsy (TLE), the most common type of refractory epilepsy in adults, are poorly understood currently. In this study, status epilepticus evoked by amygdala stimulation was used to establish rat chronic TLE model. Two months later, high-throughput sequencing was employed to investigate miRNA expression profile in rat hippocampus, and six miRNAs were confirmed to be differentially expressed. Kyoto Encyclopedia of Genes an...更多

The expression and functions of microRNAs (miRNAs) in chronic temporal lobe epilepsy (TLE), the most common type of refractory epilepsy in adults, are poorly understood currently. In this study, status epilepticus evoked by amygdala stimulation was used to establish rat chronic TLE model. Two months later, high-throughput sequencing was employed to investigate miRNA expression profile in rat hippocampus, and six miRNAs were confirmed to be differentially expressed. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that most of the target genes for these six miRNAs were associated with neuronal apoptosis. Meanwhile, the levels of miR-423-3p and miR-296-5p were correlated with the activity of caspase-3, an apoptosis indicator. Additionally, the loading of miR-423-3p was increased in RNA-induced silencing complex whilst caspase-6, a target of miR-423-3p, was reduced in chronic TLE rats. Collectively, our findings suggest that miRNAs may exert anti-apoptotic effects in chronic TLE.收起

学科

【SCI学科】MULTIDISCIPLINARY SCIENCES 【ESI学科】Multidisciplinary

影响因子

2016年:4.259;2015年:5.228;2014年:5.578;2013年:5.078;2012年:2.927

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期刊

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